Buffers
Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.
Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: Ambion
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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Intracellular Permeabilisation Buffer (10X)
Supplier: Stemcell Technologies
Ideal for the permeabilisation of fixed cells during labeling of cytokines and other cytoplasmic antigens. Intracellular Permeabilisation Buffer (10X) is uniquely formulated for optimal resolution and to reduce background and is suitable for use with fluorochrome-conjugated antibodies for flow cytometry.
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Dulbecco's Phosphate Buffered Saline (D-PBS) without Ca++ and Mg++
Supplier: Stemcell Technologies
Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.
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Buffer, TE solution (TRIS-EDTA buffer) pH 7,5
Supplier: Molekula
Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 7,5
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Buffer, TRIS solution pH 8 (1 mol/L)
Supplier: Serva
Cloning Buffer, TRIS buffer solution pH 8 (1 mol/l)
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Buffer, TRIS-glycine-SDS solution 10X concentrate (TGS buffer)
Supplier: MARKET SOURCE PART PROCESS
Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer)
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Ambion® buffer, potassium acetate, 3M™ solution, pH 5,5, RNase-free, Invitrogen™
Supplier: Ambion
Cloning Buffer, Potassium acetate buffer solution pH 5,5 (3 mol/l)
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Dulbecco's Phosphate Buffered Saline (DPBS), with 2% Fetal Bovine Serum
Supplier: Stemcell Technologies
Dulbecco's Phosphate Buffered Saline with 2% fetal bovine serum is recommended for use as a buffer to dilute or wash nucleated cells.
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Buffers for Biacore Systems
Supplier: Cytiva
Buffers developed and verified to work in Biacore™ systems.
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Buffer, TRIS-glycine solution 10X concentrate (TG buffer)
Supplier: BIO RAD
Buffer voor elektroforese, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)
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#: 20864.320
Leverancier: VWR Chemicals
#: 215-1592
Leverancier: VWR Collection
#: 83640.320
Leverancier: VWR Chemicals
#: 31770.294
Leverancier: VWR Chemicals
#: 1.00030.2500
Leverancier: Merck
#: 83645.320
Leverancier: VWR Chemicals