Buffers

Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.

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TRIS-buffered saline (TBS, 10X), with 1% Triton® X-100

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X), with 1% Triton® X-100

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Transformation buffer-2, pH 7,5

Supplier: Thermo Fisher Scientific

Cloning Buffer, Transformation buffer

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Sodium Cacodylate, 0,1 M Buffer solution, pH 6,5

Supplier: Thermo Fisher Scientific

Liquid.

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Krebs-Ringer solution, bicarbonate-buffered

Supplier: Thermo Fisher Scientific

Contains: 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, and 5,5 mM D-glucose.

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TRIS-buffered saline (TBS, 20X) pH 7,4

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 20X)

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HEPES Buffer, ES Qualified, HyClone™ AdvanceSTEM™

Supplier: HyClone products (Cytiva)

HyClone AdvanceSTEM ES Qualified HEPES provides maintenance of physiological pH in cell culture despite changes in carbon dioxide.

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Alkaline running buffer solution

Supplier: Thermo Fisher Scientific

Running Buffer, Alkaline running buffer solution

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Phosphate, 0,5M buffer solution, pH 7,5

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,5 M, pH-waarde: 7,5, Liquid

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Sodium bicarbonate, Corning®

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment

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MOPS-EDTA-sodium acetate buffer (MESA)

Supplier: Thermo Fisher Scientific

MOPS-EDTA-sodium acetate buffer (MESA), Liquid

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TRIS-buffered saline (TBS, 10X) pH 7,4

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X)

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Hybridisation buffers, formamide-free, HYB-LINK™

Supplier: G-Biosciences

Hyb-LINK™ is a high performance formamide-free hybridisation buffer solution for Northern, Southern and Dot Blots, which increases sensitivity of random-primer DNA probes.

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Buffer, sodium acetate, 1M aq, soln,, pH 4,5, RNAse free

Supplier: Thermo Fisher Scientific

Sodium acetate, 1M aq, solution, pH 4,5, RNAse free, Odourless, Liquid

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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®

Supplier: Corning

20X Saline-Sodium Citrate (SSC) buffer is used in both nucleic acid hybridization and blot transfer procedures

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GST Buffer Kit

Supplier: Cytiva

GST Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of GST-tagged proteins according to recommended protocols.

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10X Phosphate Buffered Saline (PBS), Sterile, Dulbecco's Formula, without Magnesium and Calcium

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)