Buffers
Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.
Buffers, concentrated solutions, Fluka™
Supplier: Honeywell Chemicals
pH Buffer, concentrate, pH-waarde: 4,00, ±0.02, Ampul
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Enzyme storage buffer in PBS and glycerol, autoclaved
Supplier: Thermo Fisher Scientific
Enzyme Storage Buffer in PBS and Glycerol
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LDS-sample buffer (4X), non-reducing
Supplier: Thermo Fisher Scientific
LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.
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Phosphate-buffered saline (PBS, 10X), no potassium
Supplier: Thermo Fisher Scientific
Phosphate-buffered saline (PBS), No potassium, 10X, Liquid
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Tris-HCl, 1M solution, pH 8,0, Molecular Biology Grade, Ultrapure
Supplier: Thermo Fisher Scientific
1M solution of Tris, Ultrapure in high purity distilled H₂O, adjusted to pH 8,0.
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Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: LONZA
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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Buffer, TRIS, 1,0 M Solution, pH 9,0
Supplier: Thermo Fisher Scientific
TRIS buffer solution pH 9 (1 mol/L), Odourless, Liquid
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10% SDS (Sodium Dodecyl Sulfate), Corning®
Supplier: Corning
Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.
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Maleate buffers, 0,2 M solutions, pH 5,0 - 7,0
Supplier: Thermo Fisher Scientific
Maleate buffer, 0,2 M, pH-waarde: 5,5, Colourless, Liquid
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Separating or resolving buffer (4X)
Supplier: Thermo Fisher Scientific
Separating Buffer, Separating or Resolving Buffer, 4X
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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins
Supplier: Thermo Fisher Scientific
Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific
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IEF Sample buffer (4X) pH 3 to 7 with lysine
Supplier: Thermo Fisher Scientific
Loading Buffer, IEF Sample Buffer (4X) with Lysine
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Phosphate buffer solution pH 7,00 (20 °C), Fluka™
Supplier: Honeywell Chemicals
Potassium dihydrogen phosphate / di-sodium hydrogen phosphate
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G-Biosciences Tris-EDTA-NaCl Buffer
Supplier: G-Biosciences
Tris-EDTA-NaCl buffer solution at pH 8,0.
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer) pH 8,5
Supplier: PanReac AppliChem
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer) pH 8,5
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Buffer, PBS (1X, Dulbecco's) powder pack
Supplier: PanReac AppliChem
Powder mixture to prepare PBS buffer (1X) according to Dulbecco.
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#: 20864.320
: VWR Chemicals
#: 215-1592
: VWR Collection
#: 83640.320
: VWR Chemicals
#: 31770.294
: VWR Chemicals
#: 83645.320
: VWR Chemicals