Buffers
Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.
TE buffer, (20X), pH 8,0, autoclaved
Supplier: Thermo Fisher Scientific
TE buffer concentrate, autoclaved, 20X, pH-waarde: 8,0, Liquid
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HEPES, 1,0 M buffer solutions
Supplier: Thermo Fisher Scientific
HEPES buffer solution, 1 mol/L, pH-waarde: 7,5, Liquid
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TRIS-buffered saline (TBS, 20X) pH 7,4
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 20X)
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Buffer, potassium phosphate, 0,2 M solution
Supplier: Thermo Fisher Scientific
Potassium phosphate, 0,2M buffer solution, pH 8,0, Liquid
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POPSO, 0.2 M buffer solution, pH 8.0
Supplier: Thermo Fisher Scientific
POPSO buffer solution, 0,2 M, pH-waarde: 8,0, Liquid
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Krebs-Ringer solution, bicarbonate-buffered
Supplier: Thermo Fisher Scientific
Contains: 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, and 5,5 mM D-glucose.
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Phosphate, 0,5M buffer solution, pH 7,5
Supplier: Thermo Fisher Scientific
Phosphate buffer soln, 0,5 M, pH-waarde: 7,5, Liquid
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MOPS-SDS running buffer (20X)
Supplier: Thermo Fisher Scientific
Running Buffer, MOPS-SDS running buffer (20X)
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TE buffer, pH 7,4, RNAse free
Supplier: Thermo Fisher Scientific
TE buffer is a commonly used buffer solution in procedures involving DNA or RNA. It is used for storing and diluting DNA and RNA. Treatment with TE buffer enhances the staining intensity of antibodies in the immunohistochemical detection of certain proteins.
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TRIS-buffered saline (TBS, 10X), with 1% Triton® X-100
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 10X), with 1% Triton® X-100
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TRIS-buffered saline (TBS, 10X) pH 7,4
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 10X)
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MES-SDS running buffer (20X)
Supplier: Thermo Fisher Scientific
Running Buffer, MES-SDS running buffer (20X)
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Kinase buffer II (5X)
Supplier: Thermo Fisher Scientific
Kinase buffer II (5X)
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Buffers, glycylglycine, 0,2 M solutions
Supplier: Thermo Fisher Scientific
Glycylglycine, buffer solution, 0,2 M, pH-waarde: 8,5, Colourless, Odourless, Liquid
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TE buffer, pH 8,1, RNAse free
Supplier: Thermo Fisher Scientific
Trizma is used in the formulation of buffer solutions in the pH range between 7,5 and 8,5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.
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