Buffers
Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.
Alkaline running buffer solution
Supplier: Thermo Fisher Scientific
Running Buffer, Alkaline running buffer solution
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TE buffer, (20X), pH 8,0, autoclaved
Supplier: Thermo Fisher Scientific
TE buffer concentrate, autoclaved, 20X, pH-waarde: 8,0, Liquid
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TE buffer, pH 7,4, RNAse free
Supplier: Thermo Fisher Scientific
TE buffer is a commonly used buffer solution in procedures involving DNA or RNA. It is used for storing and diluting DNA and RNA. Treatment with TE buffer enhances the staining intensity of antibodies in the immunohistochemical detection of certain proteins.
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Buffer, sodium acetate, 1M aq, soln,, pH 4,5, RNAse free
Supplier: Thermo Fisher Scientific
Sodium acetate, 1M aq, solution, pH 4,5, RNAse free, Odourless, Liquid
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TAPS, 0,2 M buffer soln., pH 8,5
Supplier: Thermo Fisher Scientific
TAPS buffer soln, 0,2 M, pH-waarde: 8,5, Liquid
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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®
Supplier: Corning
20X Saline-Sodium Citrate (SSC) buffer is used in both nucleic acid hybridization and blot transfer procedures
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Hybridisation buffers, formamide-free, HYB-LINK™
Supplier: G-Biosciences
Hyb-LINK™ is a high performance formamide-free hybridisation buffer solution for Northern, Southern and Dot Blots, which increases sensitivity of random-primer DNA probes.
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Sodium Cacodylate, 0,1 M Buffer solution, pH 6,5
Supplier: Thermo Fisher Scientific
Liquid.
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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer), Millipore®
Supplier: Merck Millipore (Calbiochem)
Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer)
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Tris Hydrochloride
Supplier: Merck Millipore (Calbiochem)
Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells.: This study highlights the application of TRIS Hydrochloride in blocking TRPM7 channels, which is significant for cell signaling and ion channel regulation in pharmacological research (Holderby & Kozak, 2024).
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Separating or resolving buffer (4X)
Supplier: Thermo Fisher Scientific
Separating Buffer, Separating or Resolving Buffer, 4X
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Tris-HCl, 1M solution, pH 8,0, Molecular Biology Grade, Ultrapure
Supplier: Thermo Fisher Scientific
1M solution of Tris, Ultrapure in high purity distilled H₂O, adjusted to pH 8,0.
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Maleate buffers, 0,2 M solutions, pH 5,0 - 7,0
Supplier: Thermo Fisher Scientific
Maleate buffer, 0,2 M, pH-waarde: 6,5, Liquid
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Buffers, MES, 0,5 M solutions
Supplier: Thermo Fisher Scientific
MES, buffer solution, 0,5 M, pH-waarde: 5,0, Colourless, Liquid
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Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula
Supplier: Thermo Fisher Scientific
Cell culture buffer, Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula
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Buffers, MOPS, 1,0 M solutions
Supplier: Thermo Fisher Scientific
MOPS buffer solution pH 7,5 (1 mol/l), pH-waarde: 7,5, Colourless, Odourless, Liquid
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