Buffers
Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.
Buffers, BIS-TRIS propane, 0,2 M solutions
Supplier: Thermo Fisher Scientific
2,2'-(Propane-1,3-diyldiimino)bis[2-(hydroxy methyl)propane-1,3-diol]
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Ionisation buffer solutions (CRMs), for AAS and ICP, Fluka™
Supplier: Honeywell Chemicals
Matrix Modifier, 2% Cesium (Cs) in 1% HNO₃
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Buffers, MES, 0,5 M solutions
Supplier: Thermo Fisher Scientific
MES, buffer solution, 0,5 M, pH-waarde: 8,0
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Maleate buffers, 0,2 M solutions, pH 5,0 - 7,0
Supplier: Thermo Fisher Scientific
Maleate buffer, 0,2 M, pH-waarde: 5,5, Colourless, Liquid
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10% SDS (Sodium Dodecyl Sulfate), Corning®
Supplier: Corning
Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.
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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins
Supplier: Thermo Fisher Scientific
Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific
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Buffer, TRIS, 1,0 M Solution, pH 9,0
Supplier: Thermo Fisher Scientific
TRIS buffer solution pH 9 (1 mol/L), Odourless, Liquid
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Tris-HCl, 1M solution, pH 8,0, Molecular Biology Grade, Ultrapure
Supplier: Thermo Fisher Scientific
1M solution of Tris, Ultrapure in high purity distilled H₂O, adjusted to pH 8,0.
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Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: LONZA
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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LDS-sample buffer (4X), non-reducing
Supplier: Thermo Fisher Scientific
LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.
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Enzyme storage buffer in PBS and glycerol, autoclaved
Supplier: Thermo Fisher Scientific
Enzyme Storage Buffer in PBS and Glycerol
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Buffers, concentrated solutions, Fluka™
Supplier: Honeywell Chemicals
pH Buffer, concentrate, pH-waarde: 10,00, ±0.05, Ampul
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TE buffer, 1X, ready to use, pH 8,0, low EDTA, Molecular Biology Grade, Affynmetrix/USB
Supplier: Thermo Fisher Scientific
TE buffer with low EDTA. Use directly to re-suspend and/or dilute purified DNA or RNA.
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Phosphate-buffered saline (PBS, 10X), no potassium
Supplier: Thermo Fisher Scientific
Phosphate-buffered saline (PBS), No potassium, 10X, Liquid
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Laemmli SDS sample buffer, reducing (4X)
Supplier: Thermo Fisher Scientific
Loading Buffer, Laemmli SDS Sample Buffer, reducing, 4X
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Phosphate-buffered saline (PBS, 10X), pH 7,4
Supplier: Thermo Fisher Scientific
Phosphate-buffered saline (PBS), 10X, pH-waarde: 7,4, Colourless, Liquid
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