Buffers
Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.
Ammonia buffer solution pH10 (20 °C) for complexometry, Fluka™
Supplier: Honeywell Chemicals
Ammonia buffer
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Solubilisation buffer, 2D-Xtract™
Supplier: G-Biosciences
A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.
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Cesium chloride - lanthanum chloride buffer, for flame AAS, TraceSELECT™, Fluka™
Supplier: Honeywell Chemicals
TraceSELECT™, for flame atomic absorption spectrometry.
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RIPA Lysis and Extraction Buffer
Supplier: G-Biosciences
A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.
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Lysis buffers, red blood cells
Supplier: G-Biosciences
These DNase- and RNase-free buffers and reagents are designed for high quality research.
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Buffer solution for chlorine determination
Supplier: Hach
For determination of chlorine by amperometric titration. APHA formulation 100 ml bottle.
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Phosphate buffer solution pH 7,00 (20 °C), Fluka™
Supplier: Honeywell Chemicals
Potassium dihydrogen phosphate / di-sodium hydrogen phosphate
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TISAB III solution (Total Ionic Strength Adjustment Buffer) for fluoride determinations
Supplier: Mettler - Toledo
TISAB III solution for fluoride determinations
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10X TAE buffer, liquid, Corning®
Supplier: Corning
Tris-Acetate-EDTA (TAE) buffer is used in DNA agarose gel electrophoresis, both in the agarose gel itself and the running buffer
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10X TBE buffer, liquid, Corning®
Supplier: Corning
TBE (Tris-Borate-EDTA) has applications in DNA and RNA agarose gel electrophoresis and polyacrylamide gel electrophoresis.
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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®
Supplier: Corning
20X Saline-Sodium Citrate (SSC) buffer is used in both nucleic acid hybridization and blot transfer procedures
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20X SSPE, liquid, Corning®
Supplier: Corning
20X Saline-Sodium-Phosphate-EDTA (SSPE) buffer has applications in nucleic acid hybridization and other molecular biology work.
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1M tris-hydrochloride buffers, Corning®
Supplier: Corning
Tris-HCl buffer has many applications in molecular biology work, including use in nucleic acid purification.
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EDTA, 0.5M pH 8.0, Corning®
Supplier: Corning
EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+. These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity. The use of EDTA therefore allows for greater control over many experiments.
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10% SDS (Sodium Dodecyl Sulfate), Corning®
Supplier: Corning
Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.
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MOPSO, 0,2 M buffer soln., pH 7,0
Supplier: Thermo Fisher Scientific
MOPSO buffer solution, 0,2 M, pH-waarde: 7,0, Liquid



