Buffers

Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.

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VWR®, Phosphate Buffered Saline (PBS), pH 7,4

Supplier: VWR Chemicals

Phosphate Buffered Saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate and potassium chloride. PBS helps to maintain a constant pH, is isotonic and non toxic to cells, and provide cells with water and essential inorganic ions. This formulation does not contain calcium and magnesium.

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VWR®, Tris-borate-EDTA (TBE) buffer

Supplier: VWR Chemicals

TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).

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VWR® UltraPure, PBS and TBS wash buffers

Supplier: VWR Chemicals

Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.

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VWR®, Buffers, Sodium acetate

Supplier: VWR Chemicals

Sodium acetate buffer D, VWR®

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J.T.Baker®, Sheath fluid, 3000/3500

Supplier: Avantor

Sheath fluid 3000/3500, J.T.Baker®

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VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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VWR® AVS TITRINORM, Bufferoplossingen, 20 °C

Supplier: VWR Chemicals

Oplossingen bereid van AnalaR® NORMAPUR®-producten voor analyses.

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VWR®, AVS TITRINORM Bufferoplossingen, CRM's, bij 25 °C

Supplier: VWR Chemicals

pH Buffer solution, Fosfaat, pH-waarde: 7,41, Referentietemperatuur: 25 °C, VWR®, kleurloos

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VWR® UltraPure, Buffer, PBS solution 10X concentrate (phosphate buffered saline)

Supplier: VWR Chemicals

Cell culture buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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VWR® AVS TITRINORM pH-bufferoplossingen in zakjes, 20 °C

Supplier: VWR Chemicals

Voor kalibratie van pH-meters.

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VWR® Tris Buffer Solution, pH 7,5 (1 mol/L), UltraPure Grade

Supplier: VWR Chemicals

TRIS buffer solution pH 7,5 (1 mol/l), VWR®

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VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer

Supplier: VWR Chemicals

TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.

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VWR® Buffer, HEPES Solution pH 7,3 (1 mol/L)

Supplier: VWR Chemicals

HEPES buffer solution pH 7.3 (1 mol/l), VWR®

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VWR®, Cell culture salt solutions, Dulbecco's Phosphate Buffered Saline (DPBS)

Supplier: VWR Chemicals

DPBS is commonly used salt solution in cell enumeration as a diluent, for rinsing cells and as a buffer in many chromatographic procedures. DPBS is also used in FACS (Fluorescence-Activated Cell Sorting) process to wash and re-suspend cells during the dissociation process, as well as for many analytical methods or biochemical treatments.