Buffers

Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.

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Western Blot Stripping Buffer, Abcam

Supplier: Abcam

Western Blot Stripping Buffer (ab282569) uniformly removes antibodies from Western blots so that the blots may be reprobed with different antibodies.

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HeLa Nuclear Extract, Abcam

Supplier: Abcam

Nuclear extract is prepared from HeLa cells, which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.

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AEC Substrate Buffer, Abcam

Supplier: Abcam

This product is manufactured by BioVision, an Abcam company and was previously called 2123 AEC Substrate Buffer.

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1X Red Blood Cell Lysis Buffer, Abcam

Supplier: Abcam

Red Blood Cell (RBC) Lysis Buffer ab286858 provides a quick and efficient method of lysing red blood cells. It is provided in a 1x concentrated format, and is also available as ab204733 in a 10x format.

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RIPA Buffer, Abcam

Supplier: Abcam

A ready-to-use buffer useful for lysing cells and tissues for radioimmunoprecipitation assay.

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Cell Lysis Buffer, Abcam

Supplier: Abcam

This product is manufactured by BioVision, an Abcam company and was previously called 1067 Cell Lysis Buffer.

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Depyrogenated Dilution Tubes

Supplier: FUJIFILM WAKO CHEMICALS

These depyrogenated dilution tubes are made of high-quality borosilicate glass depyrogenated at 250 °C.

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Gel-clot Reaction Tubes

Supplier: FUJIFILM WAKO CHEMICALS

These Gel-clot Reaction Tubes are made of high-quality borosilicate glass, depyrogenated at 250 °C. This results in an endotoxin-free tube that is designed to perform successful LAL gel clot assays with heat blocks and water baths.

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RIPA cell lysis buffer 2

Supplier: Enzo Life Sciences

RIPA cell lysis buffer 2, Enzo Life Sciences

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Wash buffer concentrate

Supplier: Enzo Life Sciences

Liquid. TRIS buffered saline containing detergents.

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Cell lysis buffer 4, (100 ml), Enzo Life Sciences

Supplier: Enzo Life Sciences

Cell lysis buffer 4, Enzo Life Sciences

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Neutralising reagent

Supplier: Enzo Life Sciences

Neutralising buffer for low pH solutions and for use with cyclic nucleotide and isoprostane kits.

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Cell lysis buffer, RIPA

Supplier: Enzo Life Sciences

The RIPA liquid cell lysis buffer composes of 50 mM TRIS-hydrogen chloride, pH 7,4, containing 150 mM sodium chloride, 1 mM EDTA, 1 mM EGTA, 1,2% Triton X-100, 0,5% sodium deoxycholate, and 0,1% SDS.

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Extraction reagents

Supplier: Enzo Life Sciences

Concentrated lysis buffer for preparation of cell and tissue extracts.

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Tween-20 solution (10%)

Supplier: Enzo Life Sciences

This Tween®-20 solution (10%) is specially designed to create the Blocking Buffer and Assay Buffer in ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol.