Buffers

Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.

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SDS-PAGE Sample Buffer, 4X, (2-Mercaptoethanol-free) for Electrophoresis

Supplier: TCI

This product contains 8% SDS, 40% glycerol, 0.02% bromophenol blue and 0.25 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol.

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G-Biosciences MOPS Running Buffers (10X)

Supplier: G-Biosciences

A zwitterionic buffer providing consistent buffering capacity in the neutral range, ideal for RNA electrophoresis.

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Buffer, TBS solution 10X concentrate (TRIS buffered saline) with phenol red, pH 7,4

Supplier: Cayman Chemical

250 mM Tris-base, pH 7,4, 1,37 M sodium chloride, containing 27 mM KCl, 0,4 mM phenol red.

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Proteometer-CV mobile phase B salts

Supplier: NOVILYTIC

1 sealed salt packet specifically formulated for use with Proteometer-CV kit.

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Cell lysis analysis kit, SideStep II

Supplier: AGILENT

Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.

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WB blocking buffer kit, TrueBlack®

Supplier: Biotium

The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).

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Cell lysis buffer 4, (100 ml), Enzo Life Sciences

Supplier: Enzo Life Sciences

Cell lysis buffer 4, Enzo Life Sciences

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Dulbecco's phosphate buffered saline (DPBS), for cell culture, powder format, HyClone™

Supplier: HyClone products (Cytiva)

Balanced salt solution used for multiple cell culture applications (powder form).

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G-Biosciences Red Blood Cell Lysis Buffer

Supplier: G-Biosciences

A red blood cell lysis buffer for the isolation of white blood cells free from interference of RBCs. The optimised concentration of ammonium chloride and enhances effectively lyses red blood cells, while preserving the integrity of the leukocytes.

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Protein blocking agent, BLOT-QuickBlocker™

Supplier: G-Biosciences

BLOT-QuickBlocker™ is a modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background.

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Fixation, (buffer)

Supplier: Enzo Life Sciences

Fixation, (buffer)

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10X RIPA Buffer, Abcam

Supplier: Abcam

10X RIPA lysis buffer (pH 7.5) is a means of cell lysis and protein solubilization for both adherent and suspension-cultured mammalian cells. - Cited in over 180 publications - Suitable for immunoprecipitation (IP), ELISA, SDS-PAGE, western blot - Effectively extracts cytoplasmic, nuclear and membrane proteins

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ES Buffer

Supplier: FUJIFILM WAKO CHEMICALS

The ES Buffer is an endotoxin-specific buffer that is ideal for untreated LAL.

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Immunoassay Blocking Buffer

Supplier: Abcam

Immunoassay blocking buffer effectively preserves the conformation and activity of dried proteins in immunoassays.

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Kinase Assay Buffer I, Abcam

Supplier: Abcam

Stock solution of buffer used for assaying kinase protein.

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Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0), Abcam

Supplier: Abcam

Antigen retrieval 100X tris-EDTA buffer (pH 9.0) enables target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. - Cited in over 20 publications - Contains 5% tris, 5% tetrasodium ethylene diamine tetraacetate, 1.5% 2-butoxyethanol - Optimized for tris-EDTA buffer (pH 9.0) treated antibodies - Contains detergent for paraffin emulsification