Buffers

Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.

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Protein Block, Abcam

Supplier: Abcam

Protein block is a serum-free blocking buffer designed to reduce non-specific background staining in IHC, ELISA, and western blot (WB). - Cited in over 90 publications - No need to match species for secondary antibodies, reducing animal serum handling - Ready-to-use – no mixing or dilution required

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Proteometer-UFT buffer packets

Supplier: NOVILYTIC

3 sealed buffer packets specifically formulated for use with Proteometer-UFT kit.

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GET buffer

Supplier: Rockland Immunochemicals

GET buffer (50 mM Glucose, 10 mM EDTA, 25 mM Tris HCl, pH 8,0) is used in effective plasmid miniprep.

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MMP-1 colorimetric assay buffer

Supplier: Enzo Life Sciences

MMP-1 colorimetric assay buffer

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TE Buffer (1X) pH 8,0 for Molecular Biology

Supplier: Apollo Scientific

Cloning Buffer, TE buffer (1X)

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Buffer solution pH 5, Acetate Buffer

Supplier: Thermo Fisher Scientific

Acetate buffer, Contains 0.7 ml glacial acetic acid and 0.6 g potassium hydroxide per litre., pH-waarde: 4,98 - 5,02 (20 °C)

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Buffer, PBS (10X, Dulbecco's) powder pack

Supplier: PanReac AppliChem

Powder mixture to prepare PBS buffer (10X) according to Dulbecco.

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G-Biosciences Phosphate Buffered Saline, Sterile (1X and 10X)

Supplier: G-Biosciences

Ready-to-use, sterile, pH adjusted blend of phosphate buffer and saline solution.

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G-Biosciences Tris Buffered Saline, 10X

Supplier: G-Biosciences

TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.

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Buffer, TBS solution 10X concentrate (TRIS buffered saline) with phenol red, pH 7,4

Supplier: Cayman Chemical

250 mM Tris-base, pH 7,4, 1,37 M sodium chloride, containing 27 mM KCl, 0,4 mM phenol red.

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Dulbecco's phosphate buffered saline (DPBS), for cell culture, powder format, HyClone™

Supplier: HyClone products (Cytiva)

Balanced salt solution used for multiple cell culture applications (powder form).

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Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0), Abcam

Supplier: Abcam

Antigen retrieval 100X tris-EDTA buffer (pH 9.0) enables target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. - Cited in over 20 publications - Contains 5% tris, 5% tetrasodium ethylene diamine tetraacetate, 1.5% 2-butoxyethanol - Optimized for tris-EDTA buffer (pH 9.0) treated antibodies - Contains detergent for paraffin emulsification

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: BIOMOL RESEARCH LABORATORIES

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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WB blocking buffer kit, TrueBlack®

Supplier: Biotium

The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).