Buffers
Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.
Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)
Supplier: Biotium
Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)
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Buffer kit for calibration of fluorescent Ca² indicators
Supplier: Biotium
Kit provides a range of calibration buffers with accurate calcium concentrations and is useful for the calibration of fluorescent Ca² indicators (1,2).
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Optimizer Buffer™ for optimal cross-linking and modification
Supplier: G-Biosciences
The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.
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G-Biosciences Sodium Chloride-Sodium Citrate Buffer (20X)
Supplier: G-Biosciences
Saline-Sodium Citrate Hybridization Buffer (20X) controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting.
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Protein blocking buffers, Superior™
Supplier: G-Biosciences
Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.
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G-Biosciences Hybridization Buffer (Hybridization Neutralizing Solution)
Supplier: G-Biosciences
Ready-to-Use: Convenient, time-saving formulation ideal for consistent experimental reproducibility.
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Protein Transfer buffers
Supplier: G-Biosciences
Efficient™ Western Transfer Buffer (20X) achieves increased protein transfer efficiency. This ready-to-use transfer buffer is prepared for optimal conductivity and efficient protein transfer, without generating excessive heat or transfer distortion.
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G-Biosciences Hybridization Buffer (Hybridization Denaturing Solution)
Supplier: G-Biosciences
Ready-to-Use: Convenient, time-saving formulation ideal for consistent experimental reproducibility.
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Caspase cell lysis buffer
Supplier: Enzo Life Sciences
Caspase cell lysis buffer, Enzo Life Sciences
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10X Blocking Buffer, Abcam
Supplier: Abcam
10X blocking buffer (pH 7.5) for serum-free blocking of non-specific antibody binding. - Cited in over 30 publications - Suitable for ELISA, in-cell ELISA, ICC/IF, western blot (WB) - 100% casein blocking as preservative
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Tissue Clearing Washing Buffer 10X - hydrophobic, Abcam
Supplier: Abcam
Tissue Clearing Washing Buffer ab243306 is part of our range of tissue clearing reagents and products that enable you to set up tissue clearing easily and quickly.
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Extraction Buffer 5X PTR, Abcam
Supplier: Abcam
Extraction buffer 5X PTR is formulated for preparing cell or tissue extracts for use in immunoassays, with added phosphatase inhibitors and protease inhibitor aprotinin. - Cited in over 20 publications - Phosphatase and protease inhibitors – contains inhibitors to preserve sample integrity - Easy to use – dilute to 1X with distilled water before use, or use with extraction enhancer buffer 50X for enhanced extraction - Compatible with a variety of sample types
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Proteometer-L starter kit part 2/2 reagent (200 assays)
Supplier: NOVILYTIC
Fluorescent reagent for the Proteometer-L Starter Kit.
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Cell Lysis Buffer, Abcam
Supplier: Abcam
ab152163 is a 10X Cell Lysis Buffer used to lyse cells under non-denaturing conditions.
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In-situ hybridisation buffer for HPV probes
Supplier: Enzo Life Sciences
In situ hybridisation buffer for HPV probes (ready-to use) provides a high quality hybridisation medium for In situ hybridisation analyses using either DNA or oligonucleotide probes labeled with biotin-, or fluorescein-modified nucleotides.
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