Buffers

Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.

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TISAB IV Solution for Determination of Fluoride, Supelco®

Supplier: Merck

Total ionic strength adjustment buffers (TISAB), is generally used for the microdetermination of fluorides.

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ISA (ionic strength adjustment solution: 1 M KCl), Supelco®

Supplier: Merck

Solution used to establish a constant ionic strength in a sample solution containing e.g. calcium for det. with ISE.

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Buffer tablets according to Weise, Sigma-Aldrich®

Supplier: Merck

Ideal for preparing buffer solution according to WEISE for staining of blood smears.

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Reagents for complexometric titration with EDTA, Supelco®

Supplier: Merck

Ammonium buffer solution for complexometry (ammonium chloride/ammonia) pH = 10 - 11, TitriPUR®

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Cesium chloride-lanthanum chloride buffer solution acc. to Schinkel for atomic absorption spectroscopy (AAS), Supelco®

Supplier: Merck

10 g/L CsCl + 100 g/L La in dilute hydrochloric acid.

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TISAB I Solution, Supelco®

Supplier: Merck

TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.

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Tris-EDTA (TE) buffer solution for molecular biology, pH 7,4, BioUltra, Supelco®

Supplier: Merck

Tris-EDTA buffer solution has been used for the dilution of DNA samples.[1] It has been used for the elution of purified vector.

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Trizmal™ Buffer, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

For use in the Naphthol AS-D Chloroacetate Esterase Kit No. 91-C.

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Citrate Buffer, Concentrate, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Citrate buffer solution, 0.38 mol/L, pH 5.4 when diluted according to procedure 386A.

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TISAB solution for Fluoride Determination, Supelco®

Supplier: Merck

TISAB solution for fluoride determination

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Cesium chloride-aluminium nitrate buffer solution acc. to Schuhknecht and Schinkel, Supelco®

Supplier: Merck

Cesium chloride-aluminium nitrate buffer solution acc. to Schuhknecht and Schinkel for the determination of sodium, potassium and lithium by flame spectrometry (enth./cont. 50 g/l CsCl + 250 g/l Al(NO₃)₃ * 9 H₂O)

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Citrate buffer, pH 6.0, 10×, antigen retriever, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Citrate buffer, antigen retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies or DNA fluorescent in situ hybridization.

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Tris-HCl buffer, pH 10, 10×, antigen retriever, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Intended for heat-induced antigen retrieval on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies in immunohistochemistry (IHC). Improves accessibility of antibodies to tissue antigens.