Buffers

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Supplier: Biowest

These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

Supplier: Rockland Immunochemicals

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 50 mM Tris HCl, 10 mM magnesium sulphate at pH 7,5 and was prepared using ultra pure reagents dissolved in pharmaceutical grade deionised water.

Supplier: Rockland Immunochemicals

Concentrate diluent buffer for DAB membrane substrate, 10X

Supplier: Rockland Immunochemicals

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 60 mM Calcium Chloride, 15% v/v Glycerol, 10mM Tris HCl at a pH of 7,5.

Supplier: Rockland Immunochemicals

EDTA is widely used for scavenging metal ion in biochemistry and molecular biology. Ion depletion is commonly used to deactivate metal-dependent enzymes, either as an assay for their reactivity or to suppress damage to DNA or proteins.

Supplier: Rockland Immunochemicals

GET buffer (50 mM Glucose, 10 mM EDTA, 25 mM Tris HCl, pH 8,0) is used in effective plasmid miniprep.

Supplier: Rockland Immunochemicals

Phosphate buffered and Tris buffered saline are suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.

Supplier: Rockland Immunochemicals

Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.

Supplier: Rockland Immunochemicals

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Rockland Immunochemicals

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Rockland Immunochemicals

Running Buffer, Tris-phosphate-EDTA 10X (TPE) (0,9 M tris-phosphate 0,01 M EDTA pH 8,0)

Supplier: Rockland Immunochemicals

Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.

Supplier: Rockland Immunochemicals

SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.