Buffers
Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.
Ambion® Buffer, TE solution (TRIS-EDTA buffer), RNase-free, Invitrogen™
Supplier: Ambion
Cloning Buffer, TE buffer solution (TRIS-EDTA buffer)
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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer) pH 8,0
Supplier: Molekula
Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer) pH 8,0
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Ambion® Buffer, TE solution (TRIS-EDTA buffer) pH 8,0, RNase-free, Invitrogen™
Supplier: Ambion
Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0
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SDS-MW gel buffer
Supplier: Beckman Coulter
Separating Buffer, SDS-MW gel buffer, proprietary formulation, 0,2% SDS
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Buffer, TRIS solution pH 7,5 (1 mol/L)
Supplier: Serva
Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)
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Buffer, TRIS solution pH 8,8 (1 mol/L)
Supplier: Serva
Cloning Buffer, TRIS buffer solution pH 8,8 (1 mol/l)
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RoboSep™-C PBS/EDTA Buffer
Supplier: Stemcell Technologies
PBS buffer containing EDTA.
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Buffer, TRIS-glycine-SDS solution 10X concentrate (TGS buffer)
Supplier: MARKET SOURCE PART PROCESS
Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer)
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Ambion® buffer, potassium acetate, 3M™ solution, pH 5,5, RNase-free, Invitrogen™
Supplier: Ambion
Cloning Buffer, Potassium acetate buffer solution pH 5,5 (3 mol/l)
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Buffer, TRIS solution pH 8 (1 mol/L)
Supplier: Serva
Cloning Buffer, TRIS buffer solution pH 8 (1 mol/l)
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Dulbecco's Phosphate Buffered Saline (DPBS), with 2% Fetal Bovine Serum
Supplier: Stemcell Technologies
Dulbecco's Phosphate Buffered Saline with 2% fetal bovine serum is recommended for use as a buffer to dilute or wash nucleated cells.
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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer)
Supplier: Serva
Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer)
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Ammonium Chloride Solution
Supplier: Stemcell Technologies
Ammonium chloride solution is recommended for the lysis of red blood cells (RBCs) in preparations of human and mouse peripheral blood, spleen or bone marrow cells. It is buffered and optimised for gentle lysis of erythrocytes, with minimal effect on leukocytes. It does not contain a fixative agent, therefore leukocytes are viable following RBC lysis.
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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer)
Supplier: Molekula
Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)
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Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: Ambion
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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Intracellular Permeabilisation Buffer (10X)
Supplier: Stemcell Technologies
Ideal for the permeabilisation of fixed cells during labeling of cytokines and other cytoplasmic antigens. Intracellular Permeabilisation Buffer (10X) is uniquely formulated for optimal resolution and to reduce background and is suitable for use with fluorochrome-conjugated antibodies for flow cytometry.



