Buffers
Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.
Fixation Buffer
Supplier: Stemcell Technologies
Ideal for fixing cells prior to permeabilisation, fixation buffer can be used with Intracellular Permeabilisation Buffer (10X) to conduct intracellular staining for flow cytometry. This fixation buffer can also be used to preserve fluorescently labeled cells prior to analysis by flow cytometry.
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ALDEFLUOR™ Assay Buffer
Supplier: Stemcell Technologies
Use ALDEFLUOR™ Assay Buffer to optimize the detection of aldehyde dehydrogenase-bright (ALDHbr) cells and maximize fluorescent signal stability during the ALDEFLUOR™ assay.
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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer) pH 8,0
Supplier: Serva
Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer) pH 8,0
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Ambion® Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l), Invitrogen™
Supplier: Ambion
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)
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Buffer, TE solution (TRIS-EDTA buffer) pH 8,0
Supplier: Molekula
Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0
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Hanks' Balanced Salt Solution, Modified without Ca++ and Mg++
Supplier: Stemcell Technologies
Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.
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Hanks' Balanced Salt Solution with 10 mM HEPES, without Phenol Red
Supplier: Stemcell Technologies
Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Molekula
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Ambion
Running Buffer, TAE buffer solution 25X concentrate (TRIS-acetate-EDTA buffer)
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: Molekula
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
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Ion pair reagents for analytical HPLC, PIC®
Supplier: WATERS
Tetrabutylammonium hydrogen sulfate, low UV reagent
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Dulbecco's Phosphate Buffered Saline (D-PBS), 10X Concentrate without Ca++ and Mg++
Supplier: Stemcell Technologies
Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.
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Buffer, PBS solution 10X concentrate (phosphate buffered saline)
Supplier: Roth Carl
Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)
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Buffer, TRIS solution pH 8 (1 mol/L)
Supplier: Serva
Cloning Buffer, TRIS buffer solution pH 8 (1 mol/l)
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Buffer, TRIS-glycine-SDS solution 10X concentrate (TGS buffer)
Supplier: MARKET SOURCE PART PROCESS
Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer)
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Ambion® buffer, potassium acetate, 3M™ solution, pH 5,5, RNase-free, Invitrogen™
Supplier: Ambion
Cloning Buffer, Potassium acetate buffer solution pH 5,5 (3 mol/l)
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