Buffers

Buffers zijn bestand tegen pH-veranderingen en beschermen monsters wanneer zuren of basen worden toegevoegd. Zowel in poeder- als vloeibare vorm zijn ze geschikt voor het efficiënt volgen van eiwitten tijdens elektroforetische isolatie en scheiding. De zoute buffers kunnen ook worden gebruikt om pH-meters te kalibreren en te standaardiseren voor nauwkeurige metingen. Blokkerende stoffen voorkomen de hechting van antilichamen aan membraanvrije plaatsen die een storende achtergrond zouden veroorzaken. De buffers zijn verkrijgbaar in verschillende concentraties, voor elke toepassing, en kunnen vloeibaar of gedehydrateerd zijn.

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer), MP Biomedicals

Supplier: MP Biomedicals

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, SSC 20X powder for molecular biology, MP Biomedicals

Supplier: MP Biomedicals

SSC buffer used in electrophoresis and in sedimentation studies of DNA-histone aggregates. SSC is used as a hybridisation buffer in immunoblotting and DNA microarray protocols.

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BIS-TRIS, 0,2 M Buffer Solutions

Supplier: Thermo Fisher Scientific

BIS-TRIS buffer solution, 0,2 M, pH-waarde: 6,5, Colourless, Odourless, Liquid

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Tris base buffer, powder, Corning®

Supplier: Corning

Tris is commonly used in buffer solutions in biochemical and molecular biological research.

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Sodium bicarbonate, Corning®

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment

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10X Tris buffered saline, liquid, Corning®

Supplier: Corning

Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range.

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TE buffer, 1X, liquid, Corning®

Supplier: Corning

Tris-EDTA (TE) buffer is commonly used in work with nucleic acids.

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Sodium acetate, Corning® 3M™

Supplier: Corning

Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.

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G-Biosciences Tris-EDTA-NaCl Buffer

Supplier: G-Biosciences

Tris-EDTA-NaCl buffer solution at pH 8,0.

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G-Biosciences Phosphate Buffer (200 mM Each)

Supplier: G-Biosciences

Water based salt solution containing sodium phosphate monobasic and dibasic at a fixed pH.

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G-Biosciences Acetate Buffer

Supplier: G-Biosciences

Acetate buffer at pH 5,0 and 5,5 are supplied as ready to use solutions that eliminate the calculation, formulation and weighing errors.

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G-Biosciences Tris Buffered Saline with Tween 20 (10X)

Supplier: G-Biosciences

A 10X concentrated Tris Buffered Saline supplemented with Tween 20.

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Krebs-Ringer solution, bicarbonate-buffered

Supplier: Thermo Fisher Scientific

Contains: 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, and 5,5 mM D-glucose.

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Krebs-Ringer solution, HEPES-buffered

Supplier: Thermo Fisher Scientific

Contains 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, 5,5 mM HEPES, and 1 mM D-glucose

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Phosphate-buffered saline (DPBS, 1X), Dulbecco's formula, without calcium, without magnesium

Supplier: Thermo Fisher Scientific

Cell culture buffer, DPBS, 1X

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G-Biosciences ACD (Acid Citrate Dextrose) Solutions for Anticoagulants

Supplier: G-Biosciences

ACD (Acid Citrate Dextrose) Solution A and ACD (Acid Citrate Dextrose) Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma.