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Supplier: G-Biosciences

GET™ Plasmid DNA Miniprep Kit isolates high quality plasmid DNA from 1 to 5 ml E. coli cultures. The kit utilises an enhanced DNA binding column to produce high yields of plasmid.

Supplier: G-Biosciences

The OmniPrep™ kit isolates high quality genomic DNA from many different species and tissue types including animal, plant, bacteria, yeast, fungi, whole blood, and cells in culture. DNA can be isolated from samples high in polysaccharides or other contaminants that are difficult to remove from the DNA preparations.

Supplier: G-Biosciences

OmniPrep™ for Fungi Kit isolates high quality genomic DNA from fungal samples.If used according to the protocols this kit purifies DNA from 1 to 2 g fungal tissues.

Supplier: G-Biosciences

Arrest™ Extraction Buffer is an optimised combination of various chaotropic agents and RNase inhibitors, which inhibits RNase rapidly. It may be used in conjunction with any RNA extraction method, including extractions based on phenol, chloroform and other organic solvents and detergents.

Supplier: G-Biosciences

GET™ CLEAN DNA uses high binding affinity GET™ spin columns to remove salts, enzymes, unincorporated nucleotides, radiolabels, and primer-dimers from any DNA preparation of 100 bp to 20 kb. GET™ spin columns has an enhanced binding affinity for DNA, thus eliminating loss or damage of DNA.

Supplier: G-Biosciences

OmniPrep™ for Tissue Kit is to specifically isolate high quality genomic DNA from tissue samples, including fresh, frozen, fixed or paraffin-embedded tissue. The kit isolates DNA from bodily fluids, including plasma, serum, amniotic fluid, semen and CSF. Also isolates high quality DNA from cultured cells, non-mammalian nucleated blood and gram-negative bacteria.

Supplier: G-Biosciences

OmniPrep™ for Mouse Tail Kit isolates high quality genomic DNA from mouse tail samples. If used according to the protocols this kit purifies DNA from 100 to 200 cm mouse tail.

Supplier: Sartorius

LentiSELECT kits offer LentiSELECT 40, LentiSELECT 500 and LentiSELECT 1000 for the purification and concentration of VSV-G pseudotyped lentivirus from 40 to 1000 ml cell culture, leading to purified infective particles. These lentivirus purification and concentration kits offer purified recombinant lentivirus particles for in-vitro transfection a fast, safe and easy to use solution. These kits replace time-consuming ultracentrifugation protocols, which typically take about one day for large sample volumes, thus reducing the purification time to only a few hours.

Supplier: Thermo Fisher Scientific

GeneJET™ Plasmid Maxiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. GeneJET™ Plasmid Maxiprep Kit utilises silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Supplier: G-Biosciences

The EZ Yeast™ Plasmid Prep Kit is designed for the rapid isolation of plasmids from yeast patches or yeast grown in small liquid cultures. The kit is adapted from the alkaline lysis of E. coli, by providing modified alkaline lysis buffers and LongLife™ Zymolyase®, a highly stable enzyme.

Supplier: Thermo Fisher Scientific

GeneJET™ Plasmid Midiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilises silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Supplier: G-Biosciences

OmniPrep™ for Yeast Kit isolates high quality genomic DNA from yeast. When used according to the recommended protocols, this kit purifies DNA from 300 ml yeast culture.

Supplier: G-Biosciences

OmniPrep™ for Plant Kit isolates high quality genomic DNA from plant samples, including fresh and frozen plant tissues. If used according to the protocols this kit purifies DNA from 20gm plant tissue.

Supplier: G-Biosciences

OmniPrep™ for Blood Kit isolates high quality genomic DNA from blood samples, including whole blood, buffy coats, packed cells and bone marrow.

Supplier: G-Biosciences

OmniTemplate™ is specifically designed for the rapid isolation of a DNA template for PCR analysis from mammalian tissue samples, blood and cell cultures. This single tube method produces a high concentration, ready-to-use supply of DNA template for microanalysis, genotyping and a multitude of other applications.

Supplier: Thermo Fisher Scientific

GeneJET™ Whole Blood Genomic DNA Purification Mini Kit utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions.

Supplier: Thermo Fisher Scientific

GeneJET™ Whole Blood RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from whole blood and related body fluids. The kit utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 15 minutes. The purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analysis.

Supplier: Merck Millipore (Novagen)

De SpinPrep™ Gel DNA Kit zorgt voor efficiënte extractie van DNA-fragmenten van 150 bp tot >12,000 bp uit agarosegels. Bij de procedure wordt gebruik gemaakt van een GelMelt™ oplossing om de gel-schijf op te lossen, gevolgd door adsorptie van het DNA in een silicamembraan in een centrifugeerkolomformaat. Na een wasstap wordt het gezuiverde DNA geëlueerd in een buffer met laag zoutgehalte. Elke centrifugeerkolom kan maximaal 20 µg DNA binden. Terugwinning is onder normale omstandigheden consistent binnen het bereik van 50 tot 90%.

Supplier: G-Biosciences

GET™ Plant DNA Template is a spin column format kit suitable for the preparation of DNA templates from plant samples. The isolated genomic DNA is suitable for PCR and other applications. GET™ Plant DNA Template purifies plant genomic DNA free from plant impurities known to inhibit downstream applications. The high yield, extracted DNA is normally >50 kb in size. The yield is ~30 µg DNA per preparation, depending on the plant source and quantity used.

Supplier: Merck Millipore (Novagen)

Pellet Paint® NF Co-Precipitant is een niet-fluorescerende drager met kleurstoflabeling die compatibel is met fluorescente sequentiebepaling. Het product vereenvoudigt een snelle verwijdering van BigDye® Terminators tijdens de alcoholneerslag van reactieproducten van cyclussequenties. Cyclussequentiereacties kunnen snel worden neergeslagen met centrifugeertijden van 10 minuten. De drager met goede visualisatie zorgt voor een eenvoudige bevestiging dat neerslag heeft plaatsgevonden. Sequentiereactieproducten worden op efficiënte wijze omgezet in korrels en terminators met kleurstoflabeling blijven achter in het supernatans tijdens alcoholneerslag gebruik makend van de standaard neerslagprotocollen van Applied Biosystems. Hersuspensie van de pellets in gedeïoniseerde formamide kan worden bevestigd door te controleren of de drager is opgelost.

Supplier: Merck Millipore (Novagen)

Pellet Paint® Co-Precipitant is een met zichtbare kleurstof gelabelde drager die specifiek is geformuleerd voor gebruik bij alcoholbezinking van nucleïnezuren. Voor de bezinking is geen incubatie bij lage temperaturen of langdurige centrifugering vereist. RNA en DNA worden efficiënt neergeslagen vanuit de meest verdunde oplossingen (2 ng/ml) en de pellet kan gemakkelijk worden herkend dankzij de felroze kleur. De pellets kunnen gemakkelijk worden gevolgd tijdens wasstappen; dit voorkomt verlies tijdens hantering en zorgt voor hersuspensie van pellets.

Supplier: Merck Millipore (Novagen)

The SpinPrep™ PCR Clean Up Kit is designed for the rapid purification of DNA amplified by PCR. The 10-minute procedure involves addition of a binding buffer followed by adsorption of the DNA to a silica membrane in a spin column format. Following a wash step, the DNA is eluted in low salt buffer. This kit removes DNA polymerises, dNTPs, salts, and >99% of primers so that they do not interfere with downstream applications such as cloning, sequencing, or labelling. PCR products from 100 to >12000 bp can be cleaned up, with recoveries of amplified DNA in the range of 60 to 90% under standard conditions.

Supplier: Thermo Fisher Scientific

GeneJET™ Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types. It utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 30 minutes following cell lysis and yields purified DNA greater than 30 kb in size. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and the age of the tissue sample. The typical yield from a standard tissue source (for example, young wheat leaves) is 30 to 32 μg from 100 mg of tissue. Isolated DNA can be used directly in PCR, qPCR, Southern blotting, and enzymatic reactions.

Supplier: G-Biosciences

G-Biosciences offers a wide selection of genomic isolation kits that purify high quality genomic DNA from a variety of sources and for a wide array of applications. G-Biosciences XIT™ DNA kits produce protein-free, high quality DNA through the principle of cell lysis, protein digestion and precipitation and genomic DNA purification. No chloroform or phenol extraction is required. High quality DNA can be isolated from sample types including: animal tissues, cells, whole blood, bacteria, buccal cells, plant tissues, mouse tail, yeast and FFPE tissue. G-Biosciences XIT™ DNA kit procedures remove contaminants and enzyme inhibitors allowing the purified DNA to be ready for immediate use for all downstream analyses. The purified DNA from G-Biosciences XIT™ DNA kits have a A₂₆₀/A₂₈₀ ratio between 1,7 and 1,9 (with the exception of the buccal cells kit with a ratio between 1,8 and 2,0) and are up to 200 kb in size.

Supplier: Cytiva

Sera-Mag Select PCR clean-up, size selection reagent combines the exceptional binding characteristics of Sera-Mag carboxyl speedbeads with an optimised binding solution in a ready to use formulation. The DNA size range isolated from the process can be tailored to suit the user’s end requirements by adjusting the amount of reagent that is mixed with a fixed volume of sample.

Supplier: MP Biomedicals

RNAintact stabilisation solution is an aqueous sample storage reagent that stabilizes and preserves RNA in samples for extended period at a wide range of storage conditions (−80 to +37 °C).

Supplier: Cytiva

Sephadex™ G-100 DNA Grade is ideal for use in preparing spin columns for DNA purification.

Supplier: Cytiva

For purification of DNA (10 bases in length) from small molecules by gel filtration.

Supplier: Cytiva

For rapid purification of labelled DNA (≥20 bases) from unincorporated labelled nucleotides in a volume of 25 to 50 μl using spin column chromatography.

Supplier: Cytiva

RNAspin Mini RNA Isolation Kit is a complete RNA purification kit designed for rapid extraction of high-quality total RNA from a wide range of sample types.