Nucleotiden

Supplier: Merck

Lipid removal agent is a synthetic adsorbent of crystalline calcium silicate hydrate which is used for removing lipids and endotoxin from plasma or aqueous solutions. It is also used for DNA purification to remove protein, RNA and genomic DNA.

Supplier: VWR Chemicals

Gebruiksklare dNTP-mengsels en dNTP-sets van moleculair-biologische kwaliteit. Het dNTP-mengsel is ontwikkeld om de onderzoekers tijd te besparen en de kans op besmetting te reduceren doordat er minder pipetteerhandelingen nodig zijn. De dNTP-oplossingen zijn tevens verkrijgbaar in sets met vier afzonderlijke dNTP's van elk 100 mM. Beide uitvoeringen zijn handig voor gebruik bij PCR-reacties, DNA-labeling en sequencing reacties.

Supplier: Biotium

Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mm.

Supplier: Biotium

Highly purified mix of dNTPs (>99% by HPLC) in 0,6 mM Tris-HCl (pH 7,5); each dNTP is at a concentration of 10 or 25 mm in the solution provided.

Supplier: Biotium

5-TAMRA-dUTP (5-Tetramethylrhodamine-dUTP) can be enzymatically incorporated into DNA to make fluorescently labeled probes, and has absorption/emission at 553/577 nm. Note: 5-TAMRA-dUTP may not be compatible with PCR labeling of DNA probes. Supplied as a 1 mM solution in Tris-HCl buffer, pH 7.5.

Supplier: TriLink BioTechnologies

N1-Methyl-Pseudouridine-5'-Triphosphate is widely used in mRNA synthesis for therapeutic and vaccine development to improve mRNA functionality. This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

Supplier: TriLink BioTechnologies

TriLink® 2'-O-Methyluridine-5'-Triphosphate

Supplier: AGILENT

The PCR grade deoxynucleotide mix includes 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR.

Supplier: TriLink BioTechnologies

TriLink® 2'-Deoxyadenosine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

Supplier: TriLink BioTechnologies

This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

Supplier: Enzo Life Sciences

Cyanine 3-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 3-dUTP can also be used for multicolor fluorescence labeling.

Supplier: Enzo Life Sciences

Cyanine 5-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 5-dUTP can also be used for multicolor fluorescence labeling.

Supplier: Enzo Life Sciences

Cyanine 3-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP .

Supplier: Enzo Life Sciences

Cyanine 5-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP.

Supplier: Enzo Life Sciences

The procedure for labeling of DNA probes with a polynucleotide 'tail' containing hapten-labeled nucleotides was developed by Enzo. In such terminal labeling reactions, terminal transferase catalyzes the addition of nucleotides to any 3'-OH terminus in a template independent manner. This rapid and convenient nonradioactive labeling procedure is free of any sequence bias that is normally observed in random priming or nick translation reactions.