Nucleotiden
Reagentia voor nucleïnezuren vormen de basis van genetische analyse en moleculaire biologie en spelen een cruciale rol van klonering tot genexpressiestudies. Ontdek onze collectie met hoge-nauwkeurigheidsenzymen voor end-point PCR, geavanceerde reagentia voor isotherme amplificatie en uitgebreide kits voor nucleïnezuurzuivering. Voor precisie in kwantitatieve assays biedt onze selectie qPCR- en RT-qPCR-kits ongeëvenaarde nauwkeurigheid. Ons portfolio bevat ook reagentia voor sequencing van de volgende generatie, die geavanceerde oplossingen bieden voor genomische sequencing en gepersonaliseerde geneeskunde.
Lipid Removal Agent (LRA), Pore size: 90 Å, powder 325 mesh, Supelco®
Supplier: Merck
Lipid removal agent is a synthetic adsorbent of crystalline calcium silicate hydrate which is used for removing lipids and endotoxin from plasma or aqueous solutions. It is also used for DNA purification to remove protein, RNA and genomic DNA.
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VWR®, dNTPs
Supplier: VWR Chemicals
Gebruiksklare dNTP-mengsels en dNTP-sets van moleculair-biologische kwaliteit. Het dNTP-mengsel is ontwikkeld om de onderzoekers tijd te besparen en de kans op besmetting te reduceren doordat er minder pipetteerhandelingen nodig zijn. De dNTP-oplossingen zijn tevens verkrijgbaar in sets met vier afzonderlijke dNTP's van elk 100 mM. Beide uitvoeringen zijn handig voor gebruik bij PCR-reacties, DNA-labeling en sequencing reacties.
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TriLink® N1-Ethylpseudouridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
A modified NTP for incorporation into messenger RNAs (mRNA) using T7 RNA Polymerase. Incorporation of N1-ethylpseudouridine can reduce the immunogenicity of the resulting mRNA.
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TriLink® 2'-Fluoro-2'-deoxyuridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dUTP is incorporated in both DNA and RNA constructs to improve in vivo stability.
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TriLink® 5-Methyluridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
TriLink® 5-Methyluridine-5'-Triphosphate
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TriLink® CleanAmp® 7-Deaza-dGTP
Supplier: TriLink BioTechnologies
CleanAmp® dNTPs are chemically-modified nucleotides designed to improve specificity, sensitivity, and robustness of your PCR.
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TriLink® N1-Methoxymethylpseudouridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
TriLink® N1-Methoxymethylpseudouridine-5'-Triphosphate
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TriLink® Cytidine-5'-Triphosphate
Supplier: TriLink BioTechnologies
This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.
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TriLink® Adenosine-5'-Triphosphate
Supplier: TriLink BioTechnologies
This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.
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TriLink® 5-Methoxyuridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.
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TriLink® Cytidine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
TriLink® Cytidine-5'-O-(1-Thiotriphosphate)
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TriLink® N1-Methyl-2'-O-Methylpseudouridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
TriLink® N1-Methyl-2'-O-Methylpseudouridine-5'-Triphosphate
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Digoxigenin-dUTP, alkali-stable
Supplier: Enzo Life Sciences
Digoxigenin-labeled nucleotide suitable for non-radioactive labeling of DNA probes.
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TriLink® Biotin-16-Aminoallyl-2'-deoxyuridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
Biotin-16-Aminoallyl-2'-deoxyuridine can be enzymatically incorporated into DNA via PCR, nick translation, primer extension using Klenow(exo-) DNA polymerase, and terminal deoxynucleotidyl transferase (TdT). Biotinylated cDNA can be prepared with this NTP using Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase.
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Cyanine 5-dUTP
Supplier: Enzo Life Sciences
Cyanine 5-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 5-dUTP can also be used for multicolor fluorescence labeling.
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TriLink® CleanAmp® 7-deaza-dGTP Mix
Supplier: TriLink BioTechnologies
CleanAmp® dNTPs are chemically-modified nucleotides designed to improve specificity, sensitivity, and robustness of your PCR.
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