Ureum

Supplier: Merck

Ureum 40% in aqueous solution, Sigma-Aldrich®

Supplier: Thermo Fisher Scientific

Ureum ≥98%

Supplier: Honeywell Chemicals

Ureum 99.0-100.5%, meets analytical specification of BP, Ph. Eur., USP, Fluka™

Supplier: Thermo Fisher Scientific

Ureum ≥99.5%, Ultrapure for molecular biology

Supplier: Honeywell Chemicals

Ureum ≥99.5%, Puriss. p.a. ACS, Reag. Ph. Eur., Fluka™

Supplier: Thermo Fisher Scientific

Ureum 99%, Ultrapure

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: MP Biomedicals

Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.

Supplier: Spectrum Chemical

Urea, FCC is a component of animal feed, providing a relatively cheap source of nitrogen to promote growth. The FCC grade meets the requirements of the Food Chemical Codex indicates and is suitable for all food, beverage, nutritional supplements and personal care applications. Spectrum Chemical offers over 300 Food grade chemical ingredients packaged in laboratory size bottles to production drum quantities and are manufactured, packaged and stored under current Good Manufacturing Practices (cGMP) per 21CFR part 211 in FDA registered and inspected facilities.

Supplier: MERCK PRODUCTION CHEMICALS

Ureum, EMPROVE® api BP, Ph. Eur., USP suitable for use as an active pharmaceutical ingredient, SAFC®

Supplier: MP Biomedicals

Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex.

Supplier: MP Biomedicals

Urea is a prinicipal protein metabolite end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle and the major product for the removal of free ammonia (NH4+) in vivo.

Supplier: MERCK PRODUCTION CHEMICALS

Ureum 98.0-102.0% (by HPLC), crystalline, EMPROVE® ESSENTIAL Ph. Eur., BP, USP, JP, SAFC®

Supplier: Cytiva

Ureum

Supplier: MERCK PRODUCTION CHEMICALS

Ureum, crystallised, EMPROVE® EXPERT Ph. Eur., BP, JP, USP, ACS voor biofarmaceutische productie, SAFC®

Supplier: MP Biomedicals

Urea is a mild agent usually used in the solubilization and denaturation of proteins.