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U hebt gezocht naar: Celcultuursupplementen en -additieven

Celcultuursupplementen en -additieven

The tissue culture media supplements and additives compensate for characteristics the base medium lacks in providing maximum cell growth conditions. Incorporating the substances during development brings stability to the cultivated cell environments for maximum activity control. The stabilizing liquids added will support cell productivity, isolation, and microorganism identification. With a variety of tissue culture supplements and additives concentrations offered, a substance possessing the correct compatibility even with sensitive mediums is guaranteed.

HAT (Hypoxanthine, Aminopterin, Thymidine), Corning®

HAT (Hypoxanthine, Aminopterin, Thymidine), Corning®

Supplier: Corning

HAT Medium (hypoxanthine-aminopterin-thymidine medium) is used in mammalian cell culture for post-fusion selection to eliminate certain types of myeloma cells

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Platen met Corning® Matrigel® matrix

Platen met Corning® Matrigel® matrix

Supplier: Corning

96-well plates, coated, PS non-sterile, flat bottom, Corning® Matrigel®

    
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Epidermal growth factor, recombinant, human, Calbiochem®

Supplier: Merck Millipore (Calbiochem‎)

Recombinant, human epidermal growth factor is a protein expressed in E. coli which is identical to human EGF except for an additional N-terminal methionine.

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Cell culture growth supplement, Trace Elements A, Corning®

Cell culture growth supplement, Trace Elements A, Corning®

Supplier: Corning

Trace Elements A provides copper, zinc, iron, and selenium

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Protease inhibitor cocktails I, animal-free

Supplier: Thermo Fisher Scientific

A 1X stock solution contains 500 μM AEBSF.HCl, 150 nM aprotinin, 1 μM E-64, 0,5 mM EDTA and 1 μM leupeptin hemisulfate. Reconstitute each vial with 1 ml water to obtain a 100X stock solution.

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Cell dissociation reagent, Corning® Cellstripper®

Cell dissociation reagent, Corning® Cellstripper®

Supplier: Corning

Cellstripper® is a non-enzymatic cell dissociation solution formulated with a proprietary mixture of chelators that gently dislodge adherent cells in tissue culture. It is an alternative to trypsin when used with either serum-free or serum-supplemented media. Because Cellstripper® is a gentler reagent than trypsin, cells can be exposed to Cellstripper for longer periods of time. Cellstripper® achieves superior results in comparison to enzymatic dissociation products.

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Serumvervanger, Corning® Nu-Serum™

Serumvervanger, Corning® Nu-Serum™

Supplier: Corning

Corning® Nu-Serum™ groeimediasupplement biedt een alternatief met een laag proteïnegehalte voor pasgeboren kalverserum, foetaal runderserum en andere sera die routinematig gebruikt worden voor celcultuur. De inhoud met een laag proteïnegehalte bevordert proteïnezuivering, virusproductie, monoklonale antilichaamproductie en screening, en verhoogt de frequentie van succesvolle transfectie van cellen.

    
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Corning® Cell-Tak™ Cell and Tissue Adhesive

Corning® Cell-Tak™ Cell and Tissue Adhesive

Supplier: Corning

Corning® Cell-Tak™ Cell and Tissue Adhesive is een samenstelling van polyfenolische proteïnen gehaald uit Mytilus edulis (zeemossel). Deze proteïnen zijn de belangrijkste bestanddelen van de lijm die afgescheiden wordt door de mossel om zich te ankeren aan vaste ondergronden in het zeemilieu. Corning® Cell-Tak™ wordt gebruikt om cellen of weefselsecties met vele soorten oppervlakken te hechten, inclusief kunststof glas, metaal, PTFE en biologische materialen. Het kan de behandeling vereenvoudigen van biologische monsters voor een aantal in vitro technieken waaronder in situ hybridisatie, immunoassays, microinjectie, immunohistochemie en verwerving van primaire cellen in cultuur. Corning® Cell-Tak™ is biocompatibel en vertoont geen soortspecificiteit.

    
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Middlebrook 7H10 OADC (Oleic Albumin Dextrose Catalase) Growth Supplement

Supplier: US Biological

Enrichment supplement recommended for the cultivation of Mycobacteria sp. OADC enrichment medium is used with Middlebrook 7H10 Mycobacteria Agar (Powder).

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Polyribonucleotide poly(I)·poly(C)

Polyribonucleotide poly(I)·poly(C)

Supplier: Cytiva

The double-stranded polyribonucleotide poly(I)·poly(C) induces interferon in rabbit cells, and there has been interest in its effectiveness for inducing human interferon in vitro.

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Cell detachment solution, Accutase, MP Biomedicals

Supplier: MP Biomedicals

Accutase solution is a trypsin replacement that contains both proteolytic and collagenolytic enzymes for cell detachment.

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Cell culture supplement, Basal Medium Eagle (BME) Vitamin Concentrate (100X), MP Biomedicals

Supplier: MP Biomedicals

Basal Medium Eagle (BME) Vitamin Concentrate (100X), without L-glutamine

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Protease inhibitor cocktail, MP Biomedicals

Supplier: MP Biomedicals

A broad spectrum protease inhibitor cocktail which covers both endopeptidases and exopeptidases, non-specific and specific. The cocktail consists of four major protease inhibitors such as AEBSF, Sodium EDTA, Leupeptin and Pepstain A.

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Protease inhibitor mix

Protease inhibitor mix

Supplier: Cytiva

Sample preparation often requires the inhibition of protease activity. Protease inhibitor mix combines competitive and non competitive protease inhibitors, which protect proteins from proteolysis during purification from animal tissues, plant tissues, yeast and bacteria.

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Trace element solution 2, MP Biomedicals

Supplier: MP Biomedicals

Trace Element Solution 2 is a 1000X sterile filtered solution, suitable for use with serum-free media; ideal for plant cell culture.

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Lymphocyte Separation Medium

Lymphocyte Separation Medium

Supplier: Tonbo Biosciences

Lymphocyte Separation Medium (LSM) is a sterile-filtered iso-osmotic solution designed for the in vitro isolation of lymphocytes from diluted whole blood.

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FastGro synthetic, animal free, chemically defined FBS replacement, sterile, MP Biomedicals

FastGro synthetic, animal free, chemically defined FBS replacement, sterile, MP Biomedicals

Supplier: MP Biomedicals

FastGro allows culturing cells in vitro without the use of serum and without any animal or human derived compound. FastGro is a fully defined additive, avoiding the need for serum, for a wide range of cell culture practices. Since FastGro is chemically defined it avoids lot-to-lot variations. All components in FastGro are highly purified and identified chemical compounds.

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BIOFLOAT™ FLEX Coating Solution

BIOFLOAT™ FLEX Coating Solution

Supplier: FACELLITATE

The BIOFLOAT™ FLEX coating solution guarantees the creation of surfaces without unspecific protein or cell binding.

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Brewers yeast, MP Biomedicals

Brewers yeast, MP Biomedicals

Supplier: MP Biomedicals

Brewers yeast is often used as a source of B-complex vitamins, chromium, and selenium. It is also can be used to bake bread, ferment wine, beer and ethanol production.

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Epidermal growth factor (EGF) from E.coli, MP Biomedicals

Supplier: MP Biomedicals

Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells.

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MEM Eagle non-essential amino acids (NEAA) concentrate (100X solution), MP Biomedicals

Supplier: MP Biomedicals

Most cells can synthesise non-essential amino acids (NEAA) through glucoaminolysis, glycolysis, or the TCA cycle. When these amino acids concentration levels are low, cells need to consume more glucose and glutamine in the media and produce excess by-products, which might affect cell growth. Adding NEAA into the cell culture medium could mitigate the potential side effects caused by self-production of these non-essential amino acids.

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Dispase (5 U/ml)

Dispase (5 U/ml)

Supplier: STEMCELL Technologies

5 U/ml dispase in Hanks' balanced salt solution.

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DNase I

DNase I

Supplier: STEMCELL Technologies

For digestion of DNA.

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Collagenase Type I

Collagenase Type I

Supplier: STEMCELL Technologies

For digestion of native collagen fibrils.

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Gentle Cell Dissociation Reagent

Gentle Cell Dissociation Reagent

Supplier: STEMCELL Technologies

cGMP, enzyme-free cell dissociation reagent.

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Collagenase A, ACF

Collagenase A, ACF

Supplier: STEMCELL Technologies

Animal component-free collagenase for the digestion of native collagen fibrils.

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DisCoZyme 2, ACF

DisCoZyme 2, ACF

Supplier: STEMCELL Technologies

Collagenase/dispase blend with high collagenase and high caseinase activity.

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Collagen Solution

Collagen Solution

Supplier: STEMCELL Technologies

For preparation of collagen gels and for coating cell culture surfaces - 35 ml.

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0.1% Gelatin in Water

0.1% Gelatin in Water

Supplier: STEMCELL Technologies

Coating for cultureware.

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CellAdhere™ Laminin-521

CellAdhere™ Laminin-521

Supplier: STEMCELL Technologies

Matrix for maintenance of human ES and iPS cells in combination with TeSR™ maintenance media.

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