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Supplier: VWR Chemicals

VWR® Bovine Serum Albumin (BSA) is used in many applications as a stabilisation agent. Albumin is used to solubilise lipids, and is also used as a blocking agent in Western blots or ELISA applications. It is commonly used in cell culture protocols mainly for protein supplementation. It can also be added to viral transport media for stabilisation.

Supplier: VWR Chemicals

Minimum Essential Medium (MEM) with Earle’s Balanced Salts is a modification of Eagle’s earlier Basal Medium (BME) which contains a higher concentration of essential nutrients. These media promote the growth of a variety of normal and transformed cells. Since they contain Earle’s Balanced Salts, they are suitable for use in atmospheres charged with CO₂ gas.

Supplier: VWR Chemicals

VWR® penicillin-streptomycin solution (100X) is a large spectrum antibiotic designed to control and destroy gram-negative and gram-positive bacteria.

Supplier: VWR Chemicals

Medium 199 was developed specifically for nutritional research of chicken fibroblasts. Today, 199 media are widely used for the maintenance of non transformed cells, vaccine and virus production and primary explants of epithelial cells. The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions, and their use in a CO₂ incubator results in a rapid drop in pH of the culture medium.

Supplier: VWR Chemicals

RPMI are general purpose enriched media. Originally formulated for suspension cultures and monolayer cultures of human leukemic cells.

Supplier: VWR Chemicals

Ham's F12 was developed for growth of primary rat hepatocytes and rat prostate epithelial cells. Also used in a clonal toxicity assay using CHO cells. It is frequently used with supplements for serum-free cultures to support the growth of cells of rodent origin, or as a cloning medium for the culture of myeloma and hybrid cells.

Supplier: VWR Chemicals

L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture.

Supplier: VWR Chemicals

McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

Supplier: VWR Chemicals

Schneider's Drosophila medium promotes rapid growth of both primary and existing cell cultures derived from Drosophila spp and several other dipterans when supplemented with 5 to 20% heat-inactivated foetal bovine serum.

Supplier: VWR Chemicals

Dulbecco's Modified Eagle Medium (DMEM) is designed to preserve and maintain the growth of a broad spectrum of mammalian cell types.

Supplier: VWR Chemicals

Lymphocyte separation media is a density gradient medium for the isolation of mononuclear cells from peripheral blood, cord blood and bone marrow.

Supplier: Biowest

McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

Supplier: HyClone products (Cytiva)

Ham's F-10 was designed for use with Chinese Hamster Ovary (CHO) cells, and has been shown to support the growth of human diploid cells, white blood cells, and primary explants from rat, rabbit, or chicken tissues. This classical media can be used for serum-free growth of some cell lines including CHO cells. Serum supplementation may or may not be necessary depending on the type of cell being cultured. Ham's F-10 contains a wide variety of components, including zinc, hypoxanthine, and thymidine and is buffered with sodium bicarbonate.

Supplier: HyClone products (Cytiva)

HyClone™ SFMTransfx-293 is a serum-free, animal derived component-free medium designed to support the growth of HEK 293 cultures and promote transfection using lipofection or similar methods. Manufactured according to cGMP guidelines.

Supplier: HyClone products (Cytiva)

HyClone™ SFM4MAb media was developed through the Metabolic Pathway Design approach to increase process yields for manufacturing of human and humanised recombinant antibodies for therapeutic use.

Supplier: HyClone products (Cytiva)

HyClone Classical Liquid Media: Iscove's Modified Dulbeccos Medium (IMDM) is manufactured using ISO 9001:2000-certified processes.

Supplier: Apollo Scientific

Contains macro/micronutrients, sucrose, agar andorganics as described by Malmgren, 1996 Plant tissue culture testedFull specification given in appendix.

Supplier: Apollo Scientific

Modified version of product number PMM519, contains 30 g/L D-Sucrose. Plant Tissue Culture Tested. Soluble in water.

Supplier: Apollo Scientific

Murashige and Skoog modified basal salts (without NH4NO3) 10×1 L

Supplier: BIOCONCEPT

Hybridoma growth media (HYGM) 7 is a serum-free, protein free, chemically defined (CD) and fully defined media that can be used for the cultivation of various different hybridomas and the production of monoclonal antibodies.

Supplier: PROVITRO

Skeletal Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human skeletal muscle cells (HSKMC). Delivered as a basal medium, it is suitable for culturing Provitro HSKMC upon the addition of supplement mix components.

Supplier: MP Biomedicals

Rich medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium.

Supplier: Apollo Scientific

Proprietary formulation.

Supplier: Apollo Scientific

Contains 600 mg/L calcium nitrate and 200 mg/L casein; without ammonium nitrate.

Supplier: Apollo Scientific

IITA Formulation. Plant tissue culture tested.

Supplier: Apollo Scientific

With the macronutrients as described by Chu (1975) andthe micronutrients and vitamins as described by Gamborg et. al., 1968 Plant tissue culture testedFull specification given in appendix.

Supplier: Biowest

In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.

Supplier: HyClone products (Cytiva)

HyClone™ DMEM high glucose liquid media supports cell growth in research, upstream process development, and cell culture manufacturing processes.

Supplier: HyClone products (Cytiva)

Dry powdered media for supporting cell culture growth and biomanufacturing processes.

Supplier: Shenandoah Biotechnology

BalanCD™ CHO Feed 3 is a chemically-defined, animal component-free feed medium designed to increase process yields of antibodies and recombinant proteins in Chinese Hamster Ovary (CHO) cells in fed-batch mode.