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Transfectiereagentia

Avantor offers transfection reagents for numerous applications, including bioproduction, in vivo work, viral production, DNA and siRNA transfection, CRISPR/Cas 9 and more. While considering a suitable transfection reagent, it is important to identify the cell type and culture conditions. Specialized transfection reagents will address rare cell cultures, neurons, and primary cells are more difficult to manipulate for porous characteristics. Avantor also offers products for electroporation and the consumables needed to support the transfection workflow.

mRNA transfection reagents, RmesFect™

mRNA transfection reagents, RmesFect™

Supplier: OZ BIOSCIENCES

RmesFect™ transfection reagent is based on the TEE-technology and is specifically designed for mRNA transfection with high efficiency and low toxicity. It is suitable for in vitro mRNA (dsRNA) transfection in a large variety of cells.

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Transfection reagent, in vivo DogtorMag™

Transfection reagent, in vivo DogtorMag™

Supplier: OZ BIOSCIENCES

In vivo DogtorMag™ transfection reagent has been designed for in vivo targeted transfection of various types of nucleic acids, such as DNA, RNA, oligonucleotides, using magnetic nanoparticles coated with synthetic lipids. DNA complexes can be easily administrated through various injection routes, such as systemic administration (intravenous, intra-artery) or local administration (intratumoral, intracerebroventricular).

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DNA-transfectiereagens, NeuroMag™

DNA-transfectiereagens, NeuroMag™

Supplier: OZ BIOSCIENCES

NeuroMag™-transfectiereagens is het eerste speciale Magnetofection™-transfectiereagens voor neuronen van 1 DIV tot 21 DIV. Het is bewezen uiterst efficiënt te zijn bij het transfecteren van een grote verscheidenheid aan primaire neuronen, zoals corticale, hippocampale, dorsale wortelganglion en motorneuronen met alle soorten nucleïnezuren.

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DNA-transfectie, LipoMag™-transfectiekit

DNA-transfectie, LipoMag™-transfectiekit

Supplier: OZ BIOSCIENCES

LipoMag™-transfectiekit combineert de efficiëntie van DreamFect™ Gold met CombiMag™ magnetische nanodeeltjes. Door gebruik te maken van Magnetofection-transfectietechnieken, bereikt de LipoMag™-kit een nog hogere transfectie-efficiëntie, vooral in primaire cellen, terwijl de toxiciteit wordt verminderd. Het is geschikt voor alle soorten nucleïnezuren zoals plasmide-DNA, gelineariseerd DNA, siRNA, oligonucleotiden, dubbelstrengs RNA, mRNA en shRNA.

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DNA-transfectiereagens, 3D-FectIN™

DNA-transfectiereagens, 3D-FectIN™

Supplier: OZ BIOSCIENCES

3D-FectIN™ is een 3D-transfectiereagens dat speciaal is ontwikkeld om cellen gekweekt in 3D-hydrogels rechtstreeks te transfecteren.

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Transfection reagent, TurboFect in-vitro

Transfection reagent, TurboFect in-vitro

Supplier: Thermo Fisher Scientific

TurboFect transfection reagent is recommended for efficient transfection of plasmid DNA. Distinct from lipid-based formulas, TurboFect is a sterile solution of a proprietary cationic polymer in water. The polymer forms positively-charged complexes with DNA that are both compact and stable. These complexes protect DNA from degradation and facilitate efficient plasmid delivery into eukaryotic cells.

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Transfection kit, BacMagic™-2

Supplier: Merck Millipore (Novagen)

The Novagen® BacMagic™-2 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

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siRNA transfection reagent, RiboJuice™

Supplier: Merck Millipore (Novagen)

RiboJuice™ siRNA Transfection Reagent delivers siRNA into a wide range of mammalian cell lines for targeted gene suppression. It is compatible with GeneJuice® Transfection Reagent for cotransfection of siRNA and plasmid DNA for normalising transfection efficiency with a reporter gene.

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DNA kit, BacMagic™-2

Supplier: Merck Millipore (Novagen)

The BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

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Transfection kit, BacMagic™

Supplier: Merck Millipore (Novagen)

The Novagen® BacMagic™-1 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

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Transfection reagent, 293-Free™

Supplier: Merck Millipore (Novagen)

293-Free™ transfection reagent has a unique polycationic liposomal formulation and is designed expressly for transfection of HEK 293 cells grown in suspension culture. It is ideal for mammalian protein production. 293-Free™ transfection reagent is provided as a sterile, ready to use solution. The 1 ml size provides enough reagent to transfect a litre of culture.

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Bipolar transfection system, HBIO AgilePulse PLUS

Bipolar transfection system, HBIO AgilePulse PLUS

Supplier: BTX

Easily perform advanced transfection protocols with multiple groups of pulses-each with separate parameters.

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Protein transfection reagent, Novagen®, ProteoJuice™

Supplier: Merck Millipore (Novagen)

ProteoJuice™ protein transfection reagent is an effective reagent for the transfection of intact functional protein and peptides into mammalian cells with minimal toxicity and broad cell specificity.

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Transfectiereagens, Insect GeneJuice®

Supplier: Merck Millipore (Novagen)

Insect GeneJuice®-transfectiereagens is speciaal geformuleerd om de transfectie van Sf9 insectencellen uit te voeren met maximale efficiëntie. Dit reagens vertoont een zeer lage toxiciteit ten opzichte van de cellen en kan worden gebruikt voor zowel korte als stabiele transfecties in serumbevattende of serumvrije media. Verder kan het reagens worden gebruikt voor de cotransfectie van transferplasmides met gelineariseerd virus DNA voor de productie van recombinante baculovirussen.

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STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers

STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers

Supplier: STEMCELL Technologies

For reverse transcription of eukaryotic mRNA before qPCR or RNA sequencing.

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ArciTect™ tracrRNA Kit

ArciTect™ tracrRNA Kit

Supplier: STEMCELL Technologies

Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing.

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ArciTect™ crRNA

ArciTect™ crRNA

Supplier: STEMCELL Technologies

Custom-designed CRISPR RNA for guide RNA generation in CRISPR-Cas9 genome editing.

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ArciTect™ Cas9 Nuclease

ArciTect™ Cas9 Nuclease

Supplier: STEMCELL Technologies

Cas9 nuclease (4 μg/μl) for the generation of double-strand breaks in CRISPR-Cas9 genome editing.

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ArciTect™ High-Fidelity DNA Polymerase Kit

ArciTect™ High-Fidelity DNA Polymerase Kit

Supplier: STEMCELL Technologies

Polymerase, buffers, and dNTPs for high-fidelity PCR amplification.

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Transfection reagents, DharmaFECT® siRNA

Supplier: HORIZON DISCOVERY

A panel of four transfection reagents optimised for the efficient delivery of siRNA into mammalian cells.

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dNTP Mix (10 mM)

dNTP Mix (10 mM)

Supplier: STEMCELL Technologies

A solution containing sodium salts of dATP, dCTP, dGTP and dTTP, each at 10 mM.

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STEMscript™ cDNA Synthesis Kit with Random Primers

STEMscript™ cDNA Synthesis Kit with Random Primers

Supplier: STEMCELL Technologies

For reverse transcription of RNA without poly(A) tails, before qPCR or RNA sequencing.

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ArciTect™ T7 Endonuclease I Kit

ArciTect™ T7 Endonuclease I Kit

Supplier: STEMCELL Technologies

For estimation of CRISPR-Cas9 genome editing efficiency.

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ArciTect™ Human HPRT Positive Control Kit

ArciTect™ Human HPRT Positive Control Kit

Supplier: STEMCELL Technologies

Positive control for CRISPR-Cas9 genome editing.

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ArciTect™ Cas9-eGFP Nuclease

ArciTect™ Cas9-eGFP Nuclease

Supplier: STEMCELL Technologies

Enhanced green fluorescent protein (eGFP)-tagged Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing.

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ArciTect™ Annealing Buffer (5X)

ArciTect™ Annealing Buffer (5X)

Supplier: STEMCELL Technologies

For guide RNA generation in CRISPR-Cas9 genome editing.

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