Running-buffers

De elektroforesebuffers handhaven een constante pH-waarde tijdens de scheiding van DNA, RNA en andere eiwitten. De oplossingen met een lage lading leveren ionen die de aangelegde stroom geleiden, waardoor verschillende moleculaire groottes met verschillende snelheden kunnen bewegen voor analyse. De preparatieve stoffen bieden extra bescherming aan eiwitten en voorkomen afbraak, wat de levensvatbaarheid van het monster en nauwkeurige testen garandeert. De buffers zijn verkrijgbaar in verschillende molecuulgewichten, zodat de meest geschikte elektroforesebuffer kan worden gekozen op basis van de specifieke macromoleculen die worden verwerkt.

75 Resultaten

Sorteren op

VWR®, MOPS 10X buffer for biotechnology

Supplier: VWR Chemicals

MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.

Expand 1 Items

VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer

Supplier: VWR Chemicals

TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.

Expand 3 Items

VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade

Supplier: VWR Chemicals

Running Buffer, TBE buffer solution 10X concentrate (Tris-borate-EDTA buffer)

Expand 1 Items

VWR® UltraPure, Tris-glycine buffers, 10X concentrate

Supplier: VWR Chemicals

Running buffers for both 1-D and 2-D protein gel electrophoresis.

Expand 2 Items

VWR® Buffer, MES-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

Expand 1 Items

Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Millipore®

Supplier: Merck

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Expand 1 Items

Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: BIOMOL RESEARCH LABORATORIES

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Expand 2 Items

G-Biosciences TAE Running Buffer (50X)

Supplier: G-Biosciences

The primary use of TAE Running Buffer is in agarose electrophoresis for the separation of nucleic acids (DNA and RNA).

Expand 2 Items

TPE buffer, 10X tris-phosphate-EDTA, pH 8

Supplier: Rockland Immunochemicals

Running Buffer, Tris-phosphate-EDTA 10X (TPE) (0,9 M tris-phosphate 0,01 M EDTA pH 8,0)

Expand 1 Items

Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: Biotium

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

Expand 1 Items

Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Rockland Immunochemicals

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Expand 1 Items

SDS-Page running gel buffers, 10X concentrate

Supplier: Rockland Immunochemicals

SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.