Loading-buffers
De elektroforesebuffers handhaven een constante pH-waarde tijdens de scheiding van DNA, RNA en andere eiwitten. De oplossingen met een lage lading leveren ionen die de aangelegde stroom geleiden, waardoor verschillende moleculaire groottes met verschillende snelheden kunnen bewegen voor analyse. De preparatieve stoffen bieden extra bescherming aan eiwitten en voorkomen afbraak, wat de levensvatbaarheid van het monster en nauwkeurige testen garandeert. De buffers zijn verkrijgbaar in verschillende molecuulgewichten, zodat de meest geschikte elektroforesebuffer kan worden gekozen op basis van de specifieke macromoleculen die worden verwerkt.
Loading buffer, SDS 4X, for SDS-PAGE analysis of proteins, Sigma-Aldrich®
Supplier: Merck Millipore (Novagen)
Loading Buffer, 4X SDS sample buffer
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LDS-sample buffer (4X), non-reducing
Supplier: Thermo Fisher Scientific
LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.
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IEF Sample buffer (4X) pH 3 to 7 with lysine
Supplier: Thermo Fisher Scientific
Loading Buffer, IEF Sample Buffer (4X) with Lysine
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Laemmli SDS sample buffer, reducing (4X)
Supplier: Thermo Fisher Scientific
Loading Buffer, Laemmli SDS Sample Buffer, reducing, 4X
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Low pH SDS Sample Buffer
Supplier: EKSIGENT
SCIEX Low pH SDS Sample Buffer, 140 mL (100 mM Tris-HCl, pH 6.8, 1% SDS) allows you to use SCIEX’s Gold Standard CE-SDS with a low pH SDS sample buffer. Use of this buffer can increase non-reduced purity by decreasing method induced fragmentation. Less variability and lower RSDs means less troubleshooting and faster method development.
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Low pH Phosphate SDS Sample Buffer
Supplier: EKSIGENT
SCIEX Low pH SDS phosphate sample buffer, 140 ml (40 mM Phosphate, pH 6,5, 1% SDS) allows you to use SCIEX’s Gold Standard CE-SDS with a China Pharmacopeia compliant buffer.
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