Extractiebuffers
Extractiebuffers worden gebruiksklaar geleverd en zijn effectief voor het isoleren van totaal-RNA en andere eiwitten, waardoor de eiwituitslag en complexiteit van monsters uit cellen en weefsels verbeteren. Geoptimaliseerde extractiereagentia verbeteren de eiwitextractie in native of denaturerende buffers die compatibel zijn met downstream analytische technieken. Een geschikte bufferoplossing die wordt toegevoegd aan een eiwitmengsel tijdens het extractieproces kan helpen de stabiliteit van eiwitmoleculen te behouden terwijl ze worden blootgesteld aan verschillende krachten die bedoeld zijn om ze te isoleren voor onderzoek.
Research buffers and reagents
Supplier: G-Biosciences
These DNase- and RNase-free buffers and reagents are designed for high quality research.
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Protein extraction kit, Insect PE LB™
Supplier: G-Biosciences
Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.
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ReadiUse™ Bacterial Cell Lysis Buffer *5X* (10 ml)
Supplier: AAT Bioquest
Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules.
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ReadiUse™ Yeast Cell Lysis Buffer, 5X
Supplier: AAT Bioquest
ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilisation in laboratory applications.
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NucleoSpin® Kits for Genomic DNA Extraction
Supplier: MACHEREY-NAGEL
Combining selective purification technologies and chemistries, these are a comprehensive range of specialised DNA isolation kits for a broad range of diversified starting materials. The resulting high quality DNA serves as excellent basis for following demanding downstream applications.
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CHAPS lysis buffer
Supplier: Thermo Fisher Scientific
For cell lysis and protein extraction.
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RBC lysis buffer for mouse
Supplier: Thermo Fisher Scientific
RBC lysis buffer for mouse, Thermo Fisher Scientific
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Eiwitextractiereagens, BugBuster® (vrij van primaire aminen), Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.
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HEPES lysis buffer with Triton® X-100
Supplier: Thermo Fisher Scientific
HEPES lysis buffer with Triton® X-100, Thermo Fisher Scientific
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Denaturing lysis buffer
Supplier: Thermo Fisher Scientific
Denaturing lysis buffer, Thermo Fisher Scientific
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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins
Supplier: Thermo Fisher Scientific
Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific
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Cell lysis buffers
Supplier: Rockland Immunochemicals
Cell lysis buffer is used to lyse cells under non-denaturing conditions.
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Protein Extraction Reagent, BugBuster® en BugBuster® plus Benzonase®, Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® reagens voor eiwitextractie is samengesteld voor het voorzichtig openbreken van de celwand van E. coli, waarbij de oplosbare eiwitten worden vrijgemaakt. Het biedt een eenvoudig, snel en goedkoop alternatief voor mechanische methoden, zoals de French Press of sonicatie, voor het vrijmaken van tot expressie gebracht eiwit als voorbereiding van purificatie of andere toepassingen. Het reagens bevat een mengsel van detergentia dat de celwand kan perforeren zonder oplosbare eiwitten te denatureren.
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T-PER™ Tissue Protein Extraction Reagent
Supplier: Thermo Fisher Scientific
Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.
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RBC lysis buffer for human
Supplier: Thermo Fisher Scientific
RBC lysis buffer for human, Thermo Fisher Scientific
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RIPA Lysis and Extraction Buffer
Supplier: G-Biosciences
A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.
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