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Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce luciferase cell lysis buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.

Supplier: AAT BIOQUEST

ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilisation in laboratory applications.

Supplier: AAT BIOQUEST

Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules.

Supplier: OMEGA BIO-TEK

SP2 buffer, Omega Bio-Tek

Supplier: Thermo Fisher Scientific

The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

Supplier: G-Biosciences

FOCUS™ Extraction Buffers are experimentally optimised concentrations of critical agents, buffering and stabilising agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulphobetaines (SB). They are designed to produce optimal protein extraction and improved spot resolution for 2D gel analysis.

Supplier: Thermo Fisher Scientific

The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

Supplier: Thermo Fisher Scientific

This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.

Supplier: Thermo Fisher Scientific

This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

Supplier: G-Biosciences

Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.

Supplier: Thermo Fisher Scientific

HEPES lysis buffer with Triton® X-100, Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

Denaturing lysis buffer, Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

RBC lysis buffer for mouse, Thermo Fisher Scientific

Supplier: Merck Millipore (Novagen)

BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

Supplier: Thermo Fisher Scientific

For cell lysis and protein extraction.

Supplier: Rockland Immunochemicals

Cell lysis buffer is used to lyse cells under non-denaturing conditions.

Supplier: Thermo Fisher Scientific

Triton® X-100 lysis buffer, pH 7.4, Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer, Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer with glycerol (2X), Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.

Supplier: Thermo Fisher Scientific

RBC lysis buffer for human, Thermo Fisher Scientific

Supplier: Merck Millipore (Novagen)

BugBuster® reagens voor eiwitextractie is samengesteld voor het voorzichtig openbreken van de celwand van E. coli, waarbij de oplosbare eiwitten worden vrijgemaakt. Het biedt een eenvoudig, snel en goedkoop alternatief voor mechanische methoden, zoals de French Press of sonicatie, voor het vrijmaken van tot expressie gebracht eiwit als voorbereiding van purificatie of andere toepassingen. Het reagens bevat een mengsel van detergentia dat de celwand kan perforeren zonder oplosbare eiwitten te denatureren.

Supplier: Thermo Fisher Scientific

Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific

Supplier: Merck Millipore (Novagen)

BugBuster HT-eiwitextractiereagens combineert BugBuster-eiwitextractiereagens en Benzonase®-nuclease in één handig reagens. BugBuster HT maakt een einde aan veelvoorkomende problemen die optreden bij traditionele cellysisprocedures. Oplosbare eiwitten worden zorgvuldig geëxtraheerd uit E. coli zonder blootstelling aan hitte of oxidatieschade en de viscositeit wordt geminimaliseerd met restrictie van nucleïnezuren in één stap. Het resulterende eiwitextract kan eenvoudig worden gefractioneerd met conventionele zuiveringstechnieken. BugBuster HT is bij uitstek geschikt voor eiwitzuiveringen met een hoog doorvoervolume.

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

MNE buffer, pH 6.5, Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer, low salt, Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

HEPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific

Supplier: G-Biosciences

A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.

Supplier: Merck Millipore (Novagen)

BugBuster® 10X is een geconcentreerde formule van de gepatenteerde reinigingsmiddelen die in BugBuster® worden gebruikt zonder de toevoeging van zouten of buffercomponenten. Geconcentreerd BugBuster® biedt een flexibel alternatief voor de gebruiksklare 1X BugBuster®, waarbij de gebruiker het reagens zelf kan verdunnen of buffercomponenten kan toevoegen. BugBuster® 10X biedt alle voordelen van standaard-BugBuster® plus de vrijheid om de vereiste pH, reagensconcentratie en bufferadditieven te bepalen voor de maximale extractie en activiteit van uw doeleiwit.