Eiwitzuivering
Eiwitzuiveringssystemen zijn bedoeld om één of meerdere eiwitten uit een complexe mix te isoleren. Ze werken met cellen, weefsels of complete organismen en zuiveren via vooraf gedefinieerde startpunten en bewerkbare methodesjablonen. Met één klik biedt de compact ontworpen apparatuur realtime runcontrole en visualisatie. Geschikt voor gebruik in koelkamers, maken de producten zuivering mogelijk van affiniteits-gelabelde eiwitten, antilichamen, ongelabelde of native eiwitten, evenals monstervoorzuivering.
J.T.Baker® BAKERBOND® PROchievA™, Affinity Chromatography Resin, Recombinant Protein A
Supplier: Avantor
The J.T.Baker® BAKERBOND® PROchievA™ protein A resin offers superior dynamic binding capacity with excellent alkaline stability linked to Avantor's proprietary ligand.
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Lectin from Triticum vulgaris, immobilized on Agarose CL-4B, for affinity chromatography, Supelco®
Supplier: Merck
50% aqueous suspension in 1 M NaCl.
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Ion exchange chromatography media, Ion Exchanger I
Supplier: Merck
Ion Exchanger I is a strong acid cation exchangers widely used in separation of noble earths and amino acids, water treatment, analysis and food industry. The bond strength of equivalent ions increases with decreasing diameter of the hydrated ion.
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Ondersteunde vloeistofextractieplaten, J.T.Baker® BAKERBOND®
Supplier: Avantor
J.T.Baker® BAKERBOND®-ondersteunde platen voor vloeistofextractie (SLE) zijn een ideaal en sneller alternatief voor traditionele vloeistof-vloeistofextractie en bieden uitstekende terugwinning van analyten uit biologische matrices.
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Eiwitneerslagplaten, J.T.Baker® BAKERBOND®
Supplier: Avantor
Dankzij de J.T.Baker® BAKERBOND® eiwitneerslagplaten kunnen eiwitten snel uit biologische monsters worden verwijderd.
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Immunoprecipitation kit, Abcam
Supplier: Abcam
Abcam's Immunoprecipitation Kit (ab206996) can be used to perform immunoprecipitation (IP) and Co-IP for functional studies of immunoprecipitated proteins/complexes and SDS-PAGE or western blot analysis of immunoprecipitated proteins and complexes.
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HRP Conjugate Stabilizer - LifeXtend, Abcam
Supplier: Abcam
HRP Conjugate Stabilizer - LifeXtend (ab270548) is a proprietary multi-component reagent system that protects antibody–HRP conjugates from instability, microbrial attack, and denaturation, thus ensuring the best possible performance in experiments performed at room temperature.
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GTP-Agarose, Abcam
Supplier: Abcam
GTP-Agarose (ab270533) comprises GTP attached to agarose beads.
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Mouse TCS Antibody Purification Kit, Abcam
Supplier: Abcam
Efficient mouse antibody purification from tissue culture supernatants.
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Affinity purification Streptavidin Resin and buffers
Supplier: G-Biosciences
Streptavidin Resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. Biotin, a 244 Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹⁵ M⁻¹) and streptavidin (Ka=10¹⁵ M⁻¹). Biotin and (strept)avidin interaction is rapid and once the bond is established it can survive up to 3M guanidinehydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH 1,5 or by autoclaving. The biotinylated molecules are efficiently probed with avidin or streptavidin conjugated to reporter molecules, such as peroxidases or phosphatases.
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BSA Removal Kit (Gold/Europium labelling), Abcam
Supplier: Abcam
Our BSA Removal Kit - Nanoparticles (ab204912) is a simple one-step, 10 minute method that effectively separates BSA from the antibody.
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Hydrofobe interactiechromatografiemedia, J.T. Baker®, BAKERBOND®
Supplier: Avantor
Media op silicabasis die worden gebruikt voor de zuivering van eiwitten op basis van differentiële zoutgeïnduceerde hydrofobiciteit. De gemengde modus biedt een unieke selectiviteit met zijn hydrofobe interactie en ionenwisselfunctie.
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Antibody Purification Kit (Gold/Europium labelling), Abcam
Supplier: Abcam
Antibody Purification Kit - Nanoparticles (ab204909) removes common buffer constituents found in commercial antibodies.
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Protein G
Supplier: Rockland Immunochemicals
Protein G is a surface protein of two groups of streptococcal bacteria that has ability to bind immunoglobulins and also to albumins. Protein G are prepared from chromatographically pure protein G.
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Serum Antibody Purification Kit (Protein G), Abcam
Supplier: Abcam
Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species.
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Jacalin for IgA purification
Supplier: G-Biosciences
Jacalin, or Artocarpus integrifolia lectin, is a tetrameric two-chain lectin with a molecular weight of 66 kDa. It is a α-D-galactose binding lectin purified from jack fruit (Artocarpus integrifolia) seeds.
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