Celcultuurmedia
Celcultuurmedia behouden de juiste pH en ondersteunen de groei van veel verschillende celtypen. De formulering van het medium bevat aminozuren, vitaminen, glucose, anorganische zouten en andere groeifactoren die ontworpen zijn om de groei van cellen of micro-organismen te ondersteunen.
VWR®, Cell culture media, Minimum Essential Medium (MEM)
Supplier: VWR Chemicals
Minimum Essential Medium (MEM) with Earle’s Balanced Salts is a modification of Eagle’s earlier Basal Medium (BME) which contains a higher concentration of essential nutrients. These media promote the growth of a variety of normal and transformed cells. Since they contain Earle’s Balanced Salts, they are suitable for use in atmospheres charged with CO₂ gas.
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VWR®, Bovine serumalbumine (BSA)
Supplier: VWR Chemicals
VWR® Bovine Serum Albumin (BSA) wordt in veel toepassingen gebruikt als stabilisator. Albumine wordt gebruikt om lipiden op te lossen en wordt ook gebruikt als blokkerend middel bij Western blots of ELISA-toepassingen. Het wordt veel gebruikt in celkweekprotocollen, voornamelijk voor eiwitsuppletie. Kan ook worden toegevoegd aan virale transportmedia voor stabilisatie.
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VWR®, Cell culture media, Schneider's Drosophila medium
Supplier: VWR Chemicals
Schneider's Drosophila medium promotes rapid growth of both primary and existing cell cultures derived from Drosophila spp and several other dipterans when supplemented with 5 to 20% heat-inactivated foetal bovine serum.
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VWR®, Lymphocyte separation media
Supplier: VWR Chemicals
Lymphocyte separation media is a density gradient medium for the isolation of mononuclear cells from peripheral blood, cord blood and bone marrow.
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VWR®, Cell culture media, Dulbecco's Modified Eagles Medium (DMEM)
Supplier: VWR Chemicals
Dulbecco's Modified Eagle Medium (DMEM) is designed to preserve and maintain the growth of a broad spectrum of mammalian cell types.
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VWR®, Penicillin-streptomycin solution (100X)
Supplier: VWR Chemicals
VWR® penicillin-streptomycin solution (100X) is a large spectrum antibiotic designed to control and destroy gram-negative and gram-positive bacteria.
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VWR®, Cell culture media, Medium 199
Supplier: VWR Chemicals
Medium 199 was developed specifically for nutritional research of chicken fibroblasts. Today, 199 media are widely used for the maintenance of non transformed cells, vaccine and virus production and primary explants of epithelial cells. The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions, and their use in a CO₂ incubator results in a rapid drop in pH of the culture medium.
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VWR®, Cell culture media, RPMI 1640
Supplier: VWR Chemicals
RPMI are general purpose enriched media. Originally formulated for suspension cultures and monolayer cultures of human leukemic cells.
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VWR®, Cell culture media, McCoy's 5A medium
Supplier: VWR Chemicals
McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.
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VWR®, L-Glutamine
Supplier: VWR Chemicals
L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture.
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VWR®, Celkweekmedium, Ham's F12
Supplier: VWR Chemicals
De F12 van Ham is ontwikkeld voor de groei van primaire rattenhepatocyten en rattenprostaat-epitheelcellen. Ook gebruikt in een klonale toxiciteitstest met CHO-cellen. Ze worden vaak gebruikt met supplementen voor serumvrije culturen ter ondersteuning van de groei van cellen van knaagdieroorsprong, of als kloonmedium voor de kweek van myeloom- en hybride cellen.
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Cell culture media, McCoy's 5A medium
Supplier: Biowest
McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.
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Serum-free media, HyClone™ SFMTranfx-293 media
Supplier: HyClone products (Cytiva)
HyClone™ SFMTransfx-293 is a serum-free, animal derived component-free medium designed to support the growth of HEK 293 cultures and promote transfection using lipofection or similar methods. Manufactured according to cGMP guidelines.
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Phytotech Orchid replate medium
Supplier: Apollo Scientific
Proprietary formulation.
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Hybridoma growth and expression CD media HYGM7
Supplier: BIOCONCEPT
Hybridoma growth media (HYGM) 7 is a serum-free, protein free, chemically defined (CD) and fully defined media that can be used for the cultivation of various different hybridomas and the production of monoclonal antibodies.
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Terrestrial (Cypripedium) orchid medium, contains 600 mg/L calcium nitrate
Supplier: Apollo Scientific
Contains 600 mg/L calcium nitrate and 200 mg/L casein; without ammonium nitrate.
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