buffers

Supplier: Thermo Fisher Scientific

The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.

Supplier: Ambion

Cloning Buffer, Potassium acetate buffer solution pH 5,5 (3 mol/l)

Supplier: Stemcell Technologies

Ideal for the permeabilisation of fixed cells during labeling of cytokines and other cytoplasmic antigens. Intracellular Permeabilisation Buffer (10X) is uniquely formulated for optimal resolution and to reduce background and is suitable for use with fluorochrome-conjugated antibodies for flow cytometry.

Supplier: Ambion

Cloning Buffer, TE Solution tampon (TRIS-EDTA Tampon)

Supplier: SERACARE LIFE SCIENCES

Convenient 20X concentrate containing imidazole-buffered saline and Tween® 20.

Supplier: Serva

Cloning Buffer, TRIS solution tampon pH 8 (1 mol/l)

Supplier: MACHEREY-NAGEL

Additional supplies and consumables for DNA and RNA extraction kits.

Supplier: Molekula

Cloning Buffer, TE Solution tampon (TRIS-EDTA Tampon) pH 8,0

Supplier: MACHEREY-NAGEL

Additional supplies and consumables for DNA and RNA extraction kits.

Supplier: Ambion

Cloning Buffer, TE Solution tampon (TRIS-EDTA Tampon) pH 8,0

Supplier: Molekula

Cloning Buffer, TE Solution tampon (TRIS-EDTA Tampon) pH 7,5

Supplier: G-Biosciences

ACD (Acid Citrate Dextrose) Solution A and ACD (Acid Citrate Dextrose) Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma.

Supplier: Serva

Cloning Buffer, TRIS solution tampon pH 7,5 (1 mol/l)

Supplier: Serva

Cloning Buffer, TRIS solution tampon pH 8,8 (1 mol/l)

Supplier: HORIBA

Tampon pH 7,00 (6 flacons de 14 ml par paquet), Flacon

Supplier: Thermo Fisher Scientific

The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.