buffers
Résistant aux variations de pH, les tampons protègent les échantillons lors de l'ajout d'acides ou de bases. Disponibles sous forme de poudre ou de liquide, ils permettent un suivi efficace des protéines pendant l'électrophorèse et leur séparation. Les tampons salins peuvent également servir à calibrer et à standardiser les pH-mètres pour des mesures précises. Les substances bloquantes empêchent la fixation des anticorps aux sites libres de la membrane, évitant ainsi un bruit de fond parasite. Proposés à différentes concentrations pour répondre à tous les besoins, les tampons sont disponibles sous forme liquide ou déshydratée.
Saline solutions
Supplier: BALIS
Cell culture buffer, Physiological saline solution, 9 ml, no standards
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Réactif stabilisateur E.Z.N.A.® RNALock
Supplier: OMEGA BIO-TEK
Le réactif stabilisateur E.Z.N.A.® RNALock est un réactif innovant qui lyse rapidement les cellules et les tissus pour désactiver les RNases à température ambiante. Il suffit d'immerger les échantillons, tels que les cultures cellulaires, les tissus animaux ou les globules blancs, dans le réactif stabilisateur RNALock. L'ARN cellulaire intact sera préservé même à température élevée. Les échantillons peuvent être conservés dans ce réactif pendant plus de 12 mois à -20 °C.
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L), Molecular Biology Grade
Supplier: Serva
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)
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Fixation Buffer
Supplier: Stemcell Technologies
Ideal for fixing cells prior to permeabilisation, fixation buffer can be used with Intracellular Permeabilisation Buffer (10X) to conduct intracellular staining for flow cytometry. This fixation buffer can also be used to preserve fluorescently labeled cells prior to analysis by flow cytometry.
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Subcellular Protein Fractionation Kit for Cultured Cells
Supplier: Thermo Fisher Scientific
This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.
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Nuclear Extraction Kit, Abcam
Supplier: Abcam
Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications. - Cited in over 255 publications - High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention - Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more - Fast, simple procedure that ensures optimized nuclear protein isolation
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Subcellular protein fractionation kit for tissues
Supplier: Thermo Fisher Scientific
This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.
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HEPES Buffer Solution (1 M)
Supplier: Stemcell Technologies
HEPES buffer solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture and degree of chemical definition required for the cell culture application.
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Dulbecco's Phosphate Buffered Saline (DPBS), with 2% Fetal Bovine Serum
Supplier: Stemcell Technologies
Dulbecco's Phosphate Buffered Saline with 2% fetal bovine serum is recommended for use as a buffer to dilute or wash nucleated cells.
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J.T.Baker® Cell Lysis Solution, Biotech Reagent
Supplier: Avantor
J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.
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Y-PER™ Yeast Protein Extraction Reagent
Supplier: Thermo Fisher Scientific
This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.
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Technical pH buffer solutions, HI 5000 series
Supplier: Hanna
pH Buffer solution, Valeur pH: 1,00, ±0,01, Température de réference: 25 °C, Bouteille en plastique
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ALDEFLUOR™ Assay Buffer
Supplier: Stemcell Technologies
Use ALDEFLUOR™ Assay Buffer to optimize the detection of aldehyde dehydrogenase-bright (ALDHbr) cells and maximize fluorescent signal stability during the ALDEFLUOR™ assay.
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SSC Solution tampon saline 20x concentrée (sodium citrate)
Supplier: LONZA
Tampon d'hybridation, SSC Solution tampon saline 20x concentrée (sodium citrate)
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Hank's balanced salts, MP Biomedicals
Supplier: MP Biomedicals
Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.
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