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buffers

Tampons

Résistant aux variations de pH, les tampons protègent les échantillons lors de l'ajout d'acides ou de bases. Disponibles sous forme de poudre ou de liquide, ils permettent un suivi efficace des protéines pendant l'électrophorèse et leur séparation. Les tampons salins peuvent également servir à calibrer et à standardiser les pH-mètres pour des mesures précises. Les substances bloquantes empêchent la fixation des anticorps aux sites libres de la membrane, évitant ainsi un bruit de fond parasite. Proposés à différentes concentrations pour répondre à tous les besoins, les tampons sont disponibles sous forme liquide ou déshydratée.

 
 

Buffers, TBS, 20X solution, pH 7,4, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Used in Western blotting protocols.

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TRIS solution tampon pH 8 (1 mol/l)

Supplier: Thermo Fisher Scientific

Cloning Buffer, TRIS solution tampon pH 8 (1 mol/l)

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MOPS solution tampon 10x concentrée

Supplier: LONZA

Tampon de migration, MOPS solution tampon 10x concentrée

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EDTA disodium salt solution, 0,2% in phosphate buffered saline, MP Biomedicals

Supplier: MP Biomedicals

EDTA disodium salt, (2%) solution, in phosphate buffered saline without calcium and magnesium. Used as a gentle non-enzymatic cell dissociation reagent.

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TRIS solution tampon pH 7,4 (1 mol/l)

Supplier: Thermo Fisher Scientific

Cloning Buffer, TRIS solution tampon pH 7,4 (1 mol/l)

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Protein extraction kit, Insect PE LB™

Protein extraction kit, Insect PE LB™

Supplier: G-Biosciences

Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.

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PBS, 10X solution, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Washing buffer, PBS, 10X solution, molecular biology grade, ultrapure

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Sodium bicarbonate, Corning®

Sodium bicarbonate, Corning®

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment

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Protein Block, Abcam

Supplier: Abcam

Protein block is a serum-free blocking buffer designed to reduce non-specific background staining in IHC, ELISA, and western blot (WB). - Cited in over 90 publications - No need to match species for secondary antibodies, reducing animal serum handling - Ready-to-use – no mixing or dilution required

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STERI-BUFFER

STERI-BUFFER

Supplier: VELTEK ASSOCIATES

Developed for use as a buffer solution and diluent in lab testing such a bioburden testing, pharmaceutical microbial limits testing, and AET testing and as a rinsing agent.

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HEPES Solution tampon (1 mol/L), Corning®

HEPES Solution tampon (1 mol/L), Corning®

Supplier: Corning

HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2

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Carbonate Borax Free Buffer Solution

Supplier: REAGECON

This range of Reagecon pH Buffers are sodium tetraborate (Borax) free. They are not subject to classification by EU regulatory authorities as toxic to reproduction under CLP category 1B. Similar in approach to the existing range of Reagecon Mercury Free Buffers, these Buffer Solutions have been developed to enhance user choice and allay safety concerns, without any impact on fitness for purpose or product performance.

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J.T.Baker® DILUT-IT™, tampons, acétate, solutions concentrées

J.T.Baker® DILUT-IT™, tampons, acétate, solutions concentrées

Supplier: Avantor

Spécialement développés pour les tests de dissolution des formules pharmaceutiques et la découverte de médicaments, les concentrés DILUT-IT™, faciles à utiliser, peuvent réduire le coût global de possession en laboratoire. Il suffit d'ajouter de l'eau purifiée et vous êtes prêt pour le test.

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Blocking solution, BLOK™ Casein

Blocking solution, BLOK™ Casein

Supplier: G-Biosciences

BLOK™ Casein is a purified protein, used for blocking free binding sites in Western blot, ELISA plate well, immunohistochemistry and other immunochemical applications.

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Blocking solution, BLOK™ BSA

Blocking solution, BLOK™ BSA

Supplier: G-Biosciences

BLOK™ BSA is a 10% BSA protein blocking solution.

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Solution de chargement de gel (10X)

Supplier: EDVOTEK

1 ml de concentré pour 200 échantillons.

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