buffers
Résistant aux variations de pH, les tampons protègent les échantillons lors de l'ajout d'acides ou de bases. Disponibles sous forme de poudre ou de liquide, ils permettent un suivi efficace des protéines pendant l'électrophorèse et leur séparation. Les tampons salins peuvent également servir à calibrer et à standardiser les pH-mètres pour des mesures précises. Les substances bloquantes empêchent la fixation des anticorps aux sites libres de la membrane, évitant ainsi un bruit de fond parasite. Proposés à différentes concentrations pour répondre à tous les besoins, les tampons sont disponibles sous forme liquide ou déshydratée.
Blocking buffer, fish gelatin
Supplier: Biotium
Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.
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G-Biosciences 10X Concentrated TE Buffers (Tris-EDTA Buffer)
Supplier: G-Biosciences
The tris buffering agent and EDTA metal chelating properties help protect DNA and RNA.
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PBS tablets
Supplier: PanReac AppliChem
Tablets for preparation of 100 ml phosphate buffered saline (PBS).
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Gram Negative lysis buffer
Supplier: G-Biosciences
Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.
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Buffer, PBS tablets
Supplier: PanReac AppliChem
PBS Tablets for preparation of 200 ml phosphate buffered saline (PBS). Composition of 140 mM sodium chloride, 2,7 mM potassium chloride and 10 mM phosphate.
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Nuclear Extraction Kit, Abcam
Supplier: Abcam
Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications. - Cited in over 255 publications - High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention - Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more - Fast, simple procedure that ensures optimized nuclear protein isolation
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Nuclear (Nucleic Acid-Free) Extraction Kit, Abcam
Supplier: Abcam
Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477) provides a simple and selective method for extracting nuclear proteins (nucleic-acid free) in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, or any other procedures requiring optimized nucleic acid-free nuclear proteins.
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TRIS solution tampon pH 7 (1 mol/l)
Supplier: Cayman Chemical
Cloning Buffer, TRIS solution tampon pH 7 (1 mol/l)
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TRIS solution tampon pH 7,5 (1 mol/l)
Supplier: Cayman Chemical
Cloning Buffer, TRIS solution tampon pH 7,5 (1 mol/l)
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TRIS solution tampon pH 8 (1 mol/l)
Supplier: Cayman Chemical
Cloning Buffer, TRIS solution tampon pH 8 (1 mol/l)
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Buffers, sodium phosphate buffer solutions (0,5 mol/L)
Supplier: Cayman Chemical
Sodium phosphate stock solution (0,5 mol/L), Valeur pH: 6,0
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Tampon, solution potassium phosphate 500 mM
Supplier: Cayman Chemical
Solution phosphate de potassium tampon (0,5 mol/l), Valeur pH: 6,0
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HEPES Solution tampon (1 mol/l)
Supplier: Cayman Chemical
HEPES Solution tampon (1 mol/l)
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)
Supplier: Cayman Chemical
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
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Buffer, TBS solution 10X concentrate (TRIS buffered saline) with phenol red, pH 7,4
Supplier: Cayman Chemical
250 mM Tris-base, pH 7,4, 1,37 M sodium chloride, containing 27 mM KCl, 0,4 mM phenol red.
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Dans le cas contraire, vous recevrez des recommandations génériques.



