buffers
Résistant aux variations de pH, les tampons protègent les échantillons lors de l'ajout d'acides ou de bases. Disponibles sous forme de poudre ou de liquide, ils permettent un suivi efficace des protéines pendant l'électrophorèse et leur séparation. Les tampons salins peuvent également servir à calibrer et à standardiser les pH-mètres pour des mesures précises. Les substances bloquantes empêchent la fixation des anticorps aux sites libres de la membrane, évitant ainsi un bruit de fond parasite. Proposés à différentes concentrations pour répondre à tous les besoins, les tampons sont disponibles sous forme liquide ou déshydratée.
Blocking buffers
Supplier: Rockland Immunochemicals
A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.
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Buffer HF-Improved
Supplier: Thermo Fisher Scientific
Buffer used for pretreatment of planar silicon devices when plating with nickel plating solution products 44069 and 44070.
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Triethylammonium bicarbonate buffer, for HPLC, LiChropur™, Supelco®
Supplier: Merck
1M Buffer, also for use in ion-exchange chromatography and electrophoresis.
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Blocking buffers, FirstChoice™
Supplier: G-Biosciences
FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.
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MMP-1 colorimetric assay buffer
Supplier: Enzo Life Sciences
MMP-1 colorimetric assay buffer
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Permeabilisation buffer
Supplier: Biotium
Ready-to-use permeabilisation buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.
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TE Buffer (1X) pH 8,0 for Molecular Biology
Supplier: Apollo Scientific
Cloning Buffer, TE buffer (1X)
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Tampon de migration, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Proteasome assay buffer
Supplier: Enzo Life Sciences
Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.
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Wash buffer concentrate
Supplier: Enzo Life Sciences
Liquid. TRIS buffered saline containing detergents.
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Buffer solutions, ready to use
Supplier: Thermo Fisher Scientific
pH Buffer solution, Borate, Valeur pH: 9,2, ±0,02, incolore
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Hybridisation buffers
Supplier: G-Biosciences
The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).
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IHC Wash Buffer, Enzo Life Sciences
Supplier: Enzo Life Sciences
The IHC wash buffer is a for washing and removing excess reagents during immunostaining.
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Buffer solution pH 5, Acetate Buffer
Supplier: Thermo Fisher Scientific
Acetate buffer, Contains 0.7 ml glacial acetic acid and 0.6 g potassium hydroxide per litre., Valeur pH: 4,98 - 5,02 (20 °C)
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Electrophoresis running buffers
Supplier: G-Biosciences
There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.
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