"TriLink BioTechnologies"

Supplier: TriLink BioTechnologies

The FLuc mRNA will express a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

Supplier: TriLink BioTechnologies

TriLink® 2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

TriLink's patented CleanCap® Reagent M6 [CleanCap m6AG (3′ OMe)], is designed for the co-transcriptional capping of mRNA to produce an mRNA with base-modified Cap 1. Cap-1 mRNAs have superior in vivo activity compared to Cap-0 mRNAs produced by legacy capping methods.

Supplier: TriLink BioTechnologies

mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.

Supplier: TriLink BioTechnologies

TriLink® N1-Propylpseudouridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

TriLink’s CleanCap® M6 IVT kit, high yield is a comprehensive solution for mRNA synthesis by in vitro transcription (IVT) with CleanCap® co-transcriptional capping. The kit components are sufficient for 25×100 µl or 125×20 µl reactions under the recommended protocol. When used with the pulse feed protocol, each kit is expected to yield 20 to 25 mg of capped mRNA.

Supplier: TriLink BioTechnologies

A modified NTP for incorporation into messenger RNAs (mRNA) using T7 RNA Polymerase. Incorporation of N1-ethylpseudouridine can reduce the immunogenicity of the resulting mRNA.

Supplier: TriLink BioTechnologies

 BspQI (isoschizomers: SapI, PciSI and LguI) is a Type IIS restriction enzyme known for its unique cleavage properties. It recognizes asymmetric DNA sequences, cleaves the double-stranded DNA outside of the recognition site, and generates a 5′ overhang.

Supplier: TriLink BioTechnologies

NLS-Cre Recombinase mRNA is a capped and polyadenylated messenger RNA encoding Cre recombinase fused to a nuclear localisation sequence (NLS). Cre recombinase is a tyrosine recombinase that catalyzes recombination between two loxP sites.

Supplier: TriLink BioTechnologies

This mRNA construct, produced via in vitro transcription (IVT), expresses an enhanced version of the green fluorescent protein (EGFP), originally isolated from the jellyfish Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cells, yielding bright green fluorescence with an emission peak at 509 nm.

Supplier: TriLink BioTechnologies

2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dUTP is incorporated in both DNA and RNA constructs to improve in vivo stability.

Supplier: TriLink BioTechnologies

TriLink® 5-Methyluridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

CleanAmp® dNTPs are chemically-modified nucleotides designed to improve specificity, sensitivity, and robustness of your PCR.

Supplier: TriLink BioTechnologies

TriLink® N1-Methoxymethylpseudouridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

TriLink® 7-Deaza-7-Propargylamino-2'-deoxyadenosine-5'-Triphosphate

Supplier: TriLink BioTechnologies

Inorganic Pyrophosphatase (iPPase) is an enzyme to remove pyrophosphate in IVT to drive the reaction forward.