"TriLink BioTechnologies"

Supplier: TriLink BioTechnologies

CleanScribe™ RNA Polymerase is a novel enzyme that reduces double-stranded RNA (dsRNA) formation in IVT RNA synthesis.

Supplier: TriLink BioTechnologies

TriLink® 5-Propargylamino-2'-deoxycytidine-5'-Triphosphate

Supplier: TriLink BioTechnologies

TriLink® 2'-O-Methylcytidine-5'-Triphosphate

Supplier: TriLink BioTechnologies

Renilla luciferase (Renilla Luc) is a bioluminescent reporter protein derived from Renilla reniformis (sea pansy). In the presence of the cofactor, coelenterazine, Renilla luciferase produces light. Renilla Luc mRNA can be used as an internal control in combination with FLuc mRNA to test different conditions, such as dosage or chemical modifications, and their effect on translation.

Supplier: TriLink BioTechnologies

This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

Supplier: TriLink BioTechnologies

The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

Supplier: TriLink BioTechnologies

2'-Deoxy-P-nucleoside is a degenerate base which behaves as a universal pyrimidine and therefore base pairs with both adenine and guanine. This feature makes it a powerful tool in mutagenesis studies.

Supplier: TriLink BioTechnologies

2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddCTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

Supplier: TriLink BioTechnologies

The FLuc mRNA will express a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

Supplier: TriLink BioTechnologies

TriLink® 2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

TriLink's patented CleanCap® Reagent M6 [CleanCap m6AG (3′ OMe)], is designed for the co-transcriptional capping of mRNA to produce an mRNA with base-modified Cap 1. Cap-1 mRNAs have superior in vivo activity compared to Cap-0 mRNAs produced by legacy capping methods.

Supplier: TriLink BioTechnologies

mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.

Supplier: TriLink BioTechnologies

TriLink® N1-Propylpseudouridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

TriLink’s CleanCap® M6 IVT kit, high yield is a comprehensive solution for mRNA synthesis by in vitro transcription (IVT) with CleanCap® co-transcriptional capping. The kit components are sufficient for 25×100 µl or 125×20 µl reactions under the recommended protocol. When used with the pulse feed protocol, each kit is expected to yield 20 to 25 mg of capped mRNA.

Supplier: TriLink BioTechnologies

TriLink® 7-Deaza-7-Propargylamino-2'-deoxyadenosine-5'-Triphosphate

Supplier: TriLink BioTechnologies

Inorganic Pyrophosphatase (iPPase) is an enzyme to remove pyrophosphate in IVT to drive the reaction forward.