2778 Résultats pour : "STEMCELL Technologies"

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Modulate prostaglandin metabolism with 15-hydroxyprostaglandin dehydrogenase (15-PGDH). 15-PGDH catalyses the reversible oxidation of the 15-hydroxyl group in prostaglandins, resulting in inactivated metabolites (Ensor and Tai), it can act on a variety of prostaglandins as substrates in a NAD+ dependent manner (Cho et al.). As prostaglandins can have a range of effects on cellular processes, 15-PGDH is of considerable importance in drug development. In vitro and in vivo studies suggest 15-PGDH has tumor suppressive activity and is downregulated in certain cancers (Na et al.).15-PGDH belongs to the short-chain dehydrogenases/reductases (SDR) family; its primary structure indicates 20% homology with other SDRs, with some conserved amino acid residues (Krook et al.). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain. For consistency and reproducibility across your applications, apo M from STEMCELL comes lyophilised with ≥89% purity.

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Fetuin B is thought to have functions in female fertility, metabolism, and liver disease. A protease inhibitor belonging to the fetuin family of proteins, within the cystatin superfamily, fetuin b’s structure includes two cystatin domains (CY1 and CY2) connected by a linker, with a CPDCP-trunk and a C-terminal region (CTR) (Cuppari et al.). This plasma protein originates in the liver (Denecke et al.) and acts to inhibit the activity of ovastacin, a metalloprotease that causes the zona pellucida to harden, leading to female infertility (Karmilin et al.). Fetuin B has also been reported to increase apoptosis, and inhibit migration and invasion of prostate cancer cells, in in vitro and in vivo settings (Zhan et al.). Elevated serum fetuin B has been linked to non-alcoholic fatty liver disease (NAFLD) (Zhu et al.). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain. For consistency and reproducibility across your applications, apolipoprotein H from STEMCELL comes lyophilised with ≥92% purity, and is verified by LAL analysis to ensure endotoxin levels are ≤1,0 EU/μg protein.

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Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages. In synergy with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, it also stimulates erythroid and megakaryocyte progenitors (Barreda et al.). GM-CSF is produced by multiple cell types, including stromal cells, Paneth cells, macrophages, dendritic cells (DCs), endothelial cells, smooth muscle cells, fibroblasts, chondrocytes, and Th1 and Th17 T cells (Francisco-Cruz et al.). The receptor for GM-CSF (GM-CSFR) is composed of two subunits: the cytokine-specific α subunit (GMRα; CD116) and the common subunit βc (CD131) shared with IL-3 and IL-5 receptors (Broughton et al.). GM-CSFR is expressed on hematopoietic cells, including progenitor cells and immune cells, as well as non-hematopoietic cells. Recombinant human GM-CSF (rhGM-CSF) promotes the production of myeloid cells of the granulocytic (neutrophils, eosinophils and basophils) and monocytic lineages in vivo. It has been tested for mobilisation of hematopoietic progenitor cells and for treating chemotherapy-induced neutropenia in patients. GM-CSF is able to stimulate the development of DCs that ingest, process, and present antigens to the immune system (Francisco-Cruz et al.). This product is animal component-free.

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Insulin-like growth factor-binding protein 4 (IGFBP-4) inhibits the actions of IGF-I and IGF-II. The distribution of IGFBP-4 in tissues is dependent on its glycosylation state, however it preferentially targets connective tissue (Firth and Baxter; Qin et al.). IGFBP-4 is also produced in liver, adrenal glands, Leydig cells, developing embryos, spinal cord, and thymic cortex. IGFBP-4 inhibits WNT/β-catenin signaling, which downregulates prostate cancer proliferation (Zhu et al.). In vitro studies suggest that IGFBP-4 inhibits proliferation of neuroblastoma and glioma, while promoting bone loss in osteosarcoma and multiple myeloma (Durai et al.).

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Interleukin 6 (IL-6) is a pleiotropic growth factor with the wide range of biological activities in immune regulation, hematopoiesis, and oncogenesis. IL-6 is produced by a variety of cell types including T cells, B cells, monocytes and macrophages, fibroblasts, hepatocytes, vascular endothelial cells, and various tumor cell lines. On its own or in combination with other factors such as IL-2 and interferon-γ, IL-6 stimulates the proliferation of B cells, T cells, and hybridoma cells (Hirano et al.; Mihara et al.; Tanaka et al). In combination with cytokines such as IL-3, GM-CSF and SCF, IL-6 has been shown to promote hematopoietic progenitor cell proliferation and differentiation in vitro. IL-6 signals through a cell surface type I cytokine receptor complex consisting of the ligand-binding IL-6α (CD126) and the signal-transducing gp130 subunits. The binding of IL-6 to its receptor system includes activation of JAK/STAT signaling pathway (Mihara et al.; Peters et al.; Tanaka et al.). This product is animal component-free.

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Glial cell line-derived neurotrophic factor (GDNF) is a neurotrophic factor and a member of the tumor growth factor (TGF)-beta superfamily. The GDNF family of growth factors also includes neurturin, persephin, and artemin, which have seven conserved cysteine residues called cysteine-knot (Treanor et al.). GDNF family ligands signal through binding to specific GDNF-family receptor-α (GFRα) co-receptors and activate the RET receptor tyrosine kinase (Durbec et al.). Four different forms of GFRα co-receptors have been characterized (GFRα 1-4) out of which GDNF binds specifically to GFRα1 prior to forming a complex with RET (Airaksinen and Saarma). GDNF is known to promote survival and morphological differentiation of midbrain dopaminergic neurons in both in vivo and in vitro studies and increase their high-affinity dopamine uptake (Granholm et al.; Lin et al.). GDNF has also been shown to have restorative effects on dying dopaminergic neurons in response to degenerative toxins (Aoi et al.). GDNF, together with Human Recombinant BDNF (brain-derived neurotrophic factor; Catalog #78005), BrainPhys™ Neuronal Medium (Catalog #05790), and other supplements, can be used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into neurons (Bardy et al.). This product is animal component-free.

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Indian hedgehog (IHH) is one of three proteins in the hedgehog family which includes sonic hedgehog and desert hedgehog. Hedgehog proteins are important signaling molecules, acting through patched and smoothened receptors. During embryonic development, they are expressed in the fetal lung, gut, stomach, liver, kidney, and pancreas. IHH is strongly expressed in cartilage and regions of developing bone, and its signaling regulates growth and differentiation of bone tissue (Marigo et al.; Valentini et al.; St-Jacques et al.). IHH is also involved in yolk sac vasculogenesis, indicating a role in the differentiation of epiblast cells into endothelial and red blood cells (Byrd et al.). IHH induces the expression of parathyroid hormone-related peptide (PTHrP), which negatively regulates IHH during chondrocyte differentiation and proliferation (Vortkamp et al.).

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Immunomagnetic negative selection of rat cells

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PBS buffer containing EDTA.

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Rat monoclonal antibody against mouse IL-4, unconjugated.

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Rat monoclonal antibody against mouse CD127, APC-conjugated.

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For detection and measurement of mouse interleukin 4.

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Custom-designed single guide RNA for CRISPR-Cas9 genome editing.

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The EasyEights™ EasySep™ Magnet is designed for cell separation procedures using selected EasySep™ reagents when simultaneously processing multiple samples, up to eight samples on each side, for a total of 16 samples.

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Immunomagnetic negative selection kit

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For expansion and differentiation of hPSCs to T cells.

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Kit for the detection of extracellular vesicles using CD9, CD63, and CD81 markers.

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Fetal bovine serum for cell culture and research applications.

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The RSV (HLA Class I Control) peptide pool is a lyophilised mixture of 28 peptides from respiratory syncytial virus (RSV). The pool consists of defined HLA class I-restricted T cell epitopes, and can be used as an antigen-specific positive control to stimulate T cells.

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For the detection of human anti-SARS-CoV-2 Nucleoprotein IgG antibodies.

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For detection and measurement of human ACE2.

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Serum- and BPE-free medium for differentiation of human primary bronchial epithelial cells or human airway epithelial cells to mature apical-out airway organoids.

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Conveniently and consistently add transforming growth factor beta 1 (TGF-ꞵ1) to your cultures with this cell culture-ready formulation. This human recombinant TGF-ꞵ1 is precisely reconstituted to 0,1 mg/ml in 100 mM acetic acid and requires no additional preparation, improving reproducibility. Endotoxin levels are verified, using the LAL method to ensure consistency for use across multiple applications. A member of the TGF-β superfamily, TGF-β1 regulates diverse cellular phenotypes. TGF-β1 binds to serine-threonine kinase type I and II receptors and activates signal transduction through SMAD2/3 proteins.

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Interleukin 34 (IL-34) is well known for its ability to induce the formation of colony-forming unit macrophages in human bone marrow cell cultures (Foucher et al.; Wei et al.). This dimeric glycoprotein is a member of the short-chain helical hematopoietic cytokine family (Baghdadi et al.; Foucher et al.), and exists in two isoforms that differ by a single glutamine (Chen et al.; Foucher et al; Wei et al.). IL-34 interacts with M-CSF to trigger tyrosine phosphorylation of the receptor and ERK1/2 pathways. (Wang et al.; Wei et al.). It is expressed in many tissues (heart, brain, lung, liver, kidney, thymus, testes, ovary, small intestine, prostate, and colon), with the highest expression in the spleen. In combination with RANKL, IL-34 induces osteoclast differentiation (Chen et al.; Foucher et al.). IL-34 expression is decreased in Alzheimer’s disease and atopic dermatitis, while high levels of IL-34 are found in many types of cancer correlated with poor prognosis, chronic heart failure or coronary artery disease, inflammatory bowel disease, influenza A infection, during acute liver transplant rejection or in non-alcoholic fatty liver disease, and with rheumatoid arthritis (Baghdadi et al.). It is therefore a possible pharmacological target for treating bone or inflammatory diseases (Chen et al.). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain, and the protein was purified as a homodimer consisting of 39 kDa monomers (Lin et al.).

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Human amyloid-β (1 to 42) peptide is a neurotoxic peptide fragment that can oligomerize to form amyloid plaques, thus contributing to the onset of Alzheimer's disease (Paradis et al.; Teplow; Wolfe). These neurotoxic peptide fragments also modulate the expression of Bcl-2 and Bax in human neurons (Paradis et al.).

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Differentiation kit for the generation of sensory neuronal precursors from human iPS cell-derived neural crest cells.

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Rat monoclonal IgG2b, antibody against mouse MHC Class II (I-A/I-E), FITC-conjugated.

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Serum-free neural supplement without antioxidants.

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For expansion and differentiation of human CD34+ hematopoietic progenitor cells to NK cells.

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Column-free immunomagnetic positive selection of human CD3+ and CD19+ cells (e.g. lymphocytes) cells for HLA analysis.