28505 Résultats pour : "G-Biosciences"

Supplier: G-Biosciences

Ammonium acétate solution 5M

Supplier: G-Biosciences

FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.

Supplier: G-Biosciences

This sulphhydryl coupling resin is designed for coupling of peptides and proteins to a solid 6% agarose support through free sulfhydryl groups (-SH). The iodoacetyl groups of the sulfhydryl coupling resin specifically react with free sulfhydryls to form covalent, permanent thioether bonds.

Supplier: G-Biosciences

Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.

Supplier: G-Biosciences

Potassium iodure

Supplier: G-Biosciences

Mode of action and anti-microbial spectrum streptomycin sulphate is an aminoglycoside antibiotic and has a bactericidal action against many gram-negative bacteria. It binds to the #12 protein on the ribosomal 30S subunit and inhibits protein synthesis.

Supplier: G-Biosciences

(Bis(2-[Succinimidooxycarbonyloxy]ethyl) sulfone Homobifunctional Cross Linkers.

Supplier: G-Biosciences

Tested against both sensitive and resistant cells.

Supplier: G-Biosciences

Calcium chlorure dihydraté

Supplier: G-Biosciences

N,N'-Méthylènediacrylamide, en poudre

Supplier: G-Biosciences

di-Potassium hydrogénoorthophosphate

Supplier: G-Biosciences

Protein assay, Resorufin-Casein protease substrate

Supplier: G-Biosciences

NDSB 201 is a zwitterionic compound. Unlike zwitterionic detergents, the hydrophobic group in NDSB 201 is too short to form micelles, even at 1 M concentrations. NDSB 201 can be used for the purification of proteins and solubilisation of protein samples for 2D gel electrophoresis.

Supplier: G-Biosciences

Calcium chlorure dihydraté

Supplier: G-Biosciences

BLOK™ BLOTTO is a 5% novel modified milk protein solution used for Westerns, ELISA and dot blot blocking.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

GET™ AGAROSE DNA method involves release of DNA fragments from gel pieces followed by capture of nucleic acids on the GET™ spin columns, washing, and elution of the clean nucleic acid fragments in a suitable buffer.

Supplier: G-Biosciences

TRITON™ X-100

Supplier: G-Biosciences

Centrifuge tubes 1,5 ml.

Supplier: G-Biosciences

Protein preparations specifically prepared for standardising electrophoresis methods and protocols. Substantially free from non-protein agents such as nucleic acids, detergents, salts, lipids and other common laboratory agents, the protein preparation has low conductivity (<50 μs). Supplied as dry protein pellets, simply re-hydrate and use. Proteomic protein sample controls are available for animal cells, E.coli, yeast, plant or as a control set that contains one 2 mg vial of each.

Supplier: G-Biosciences

Acrylamide (electrophoresis grade) is supplied as a powder or a 40% solution in ultrapure water. Bisacrylamide (Bis (N,N’-methylenebisacrylamide)) (electrophoresis grade) is supplied as a powder or a 2% solution in ultrapure water.

Supplier: G-Biosciences

Tween® 20 is commonly used non ionic detergent for solubilising membrane proteins during isolation of membrane-protein complexes.

Supplier: G-Biosciences

Nonidet® P 40 Substitut (NP-40)

Supplier: G-Biosciences

This variety pack contains non ionic proteomic grade detergent solutions, zwitterionic and non-detergent sulphobetaines for trial and optimisation.

Supplier: G-Biosciences

Tube-O-CONCENTRATOR™ Mini for 20 to 250 μl volumes or Medi for 0,2 to 2,5 ml volumes.

Supplier: G-Biosciences

Anti-IgG Goat Antibody (AP (Alkaline Phosphatase))

Supplier: G-Biosciences

Anti-IgG Goat Antibody (HRP (Horseradish Peroxidase))

Supplier: G-Biosciences

This lab activity is designed to study highly specific lock-key matching properties of antigen-antibody and how this highly specific interaction can be exploited as a tool for research and analysis. This study involves the use of an immunodiffusion technique in which antigen and antibody are allowed to diffuse in a solid agarose medium. When antigen and antibody meet, antigen-antibody complex is formed, which leads to precipitation. Antigen-antibody precipitate is formed in the zone where the concentration of the two matching pairs reaches an optimal known as the zone of equivalence, which results in formation of a visible opaque precipitate region in agarose medium. Those regions of precipitation can be used for determination of concentration or titer of both antigen and antibody. This Antigen-Antibody Interaction kit is a hands-on study of both Ouchterlony Double Diffusion and Radial Immunodiffusion techniques. This kit also provides additional guidance materials for teaching other types of antigen-antibody interactions concepts such as immunoelectrophoresis and immunoprecipitation.

Supplier: G-Biosciences

En biologie moléculaire, le but final est souvent l'expression d'une protéine recombinante. Les plasmides transformés peuvent servir de modèles aux bactéries pour produire la protéine. Les étudiants se forment aux promoteurs essentiels et aux autres éléments nécessaires à la réussite de l'expression des protéines dans les bactéries, y compris les différences entre expression inductible et constitutive (non régulée). Le kit d'expression d'une protéine recombinante permet aux étudiants d'exprimer une protéine de manière constitutive ou sous le contrôle d'un promoteur inductible, activé avec l'IPTG (isopropyle-bêta-D-thiogalactopyranoside).

Supplier: G-Biosciences

SATA reacts with primary amines, adding protected sulphhydryl residues. It is soluble in DMSO and the sulphhydryl group can be used in coupling reactions.