12530 Résultats pour : "ENZO LIFE SCIENCES"

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Antioxidant. SIRT1 activator.

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Produced in E. coli. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG®-tag.

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Quantitative protein standards for Western blotting internal controls or ELISA standards.

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Produced in Sf9 insect cells. Recombinant human PDE5A (phosphodiesterase 5A) is fused at the C-terminus to a His-tag.

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The adrenergic ligand library contains 84 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein-coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The adrenergic ligand library is 1 plate of the larger neurotransmitter library which contains over 650 CNS receptor ligands.

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The Screen-Well™ Phosphatase Inhibitor Library contains 33 known phosphatase inhibitors of well-defined activity. Inhibitors are supplied dissolved in DMSO at 10 mM and aliquoted into deep-well plates at either 100 µl or 500 µl per well. The library is ideal for chemical genomics, assay development, and as a reference set for secondary screening. Includes inhibitors of these important phosphatases: Calcineurin(PP2B), JSP-1, PRL-1, CD45, PP1, PRL-3, CDC25, PP2A, PTEN, and many more.

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The Screen-Well® Wnt pathway library v 2.0 is a focused collection of 71 compounds with defined and diverse Wnt pathway activity, including activators and inhibitors of Wnts, Dishevelled, GSK-3β and other Wnt pathway kinases, TCF/β-catenin, DKK, LRP, Axin, Porcupine, and more. Compounds are dissolved in DMSO at 10 mM or 1 mM and aliquoted into deep-well plates at 100 or 500 µl per well. A variety of structurally and mechanistically different compound classes are included. A useful tool for studying the roles of pro- and anti-Wnt pathway molecules in cells as well as for use in in vitro applications.

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The Screen-Well® neurotransmitter library contains 681 CNS receptor ligands in a 96-well format. Ideal for screening or identifying recombinant orphan G protein-coupled receptors, target validation, secondary screening, validating new assays, and for routine pharmacological applications.

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DNA dye

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Mitochondria dye

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Zinc dye

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beta-Amyloid dye

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Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. Like JC-1,TMRE is widely used for measuring mitochondrial membrane potential, in addition to selectively staining mitochondria. Real-time imaging of mitochondrial membrane potential in individual cardiomyocytes within perfused rat hearts has been demonstrated with this dye, using 2-photon laser-scanning microscopy.

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Calcium dye.

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pH indicator

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Membrane Potential Detector

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RG-13022 is a non-phenolic tyrphostin analog, which inhibits the EGF receptor (IC50=5 µM) and PDGF receptor tyrosine kinases, and is long-acting. It inhibits EGF-stimulated HER14 cell proliferation (IC50=1 µM) as well as tumor growth in vivo. It inhibits both EGF- and PDGF-stimulated DNA synthesis in human glioma cell lines. RG-13022 is non-phenolic and therefore possesses less antioxidant activity than other tyrphostins.

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Benzylphosphonic acid-(AM)2 is a bis-acetoxymethyl ester (AM) derivative of benzylphosphonic acid which is cell permeable and is converted to the active phosphonic acid via intracellular esterase-catalyzed cleavage of the AM esters.

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Potent and selective protein kinase C inhibitor which has been used effectively in platelets, Swiss 3T3 fibroblasts and macrophages. Inhibitory profile: PKC, IC50=0.02µM (inhibits α, ßI, ßII and γ subtypes with similar potency); PKA, IC50=2.0µM; phosphorylase kinase, IC50=0.7µM; insulin, EGF and PDGF receptor tyrosine kinases are not inhibited at concentrations up to 50µM.

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Small molecule STAT3-specific inhibitor which blocks activation, dimerization and nuclear translocation via binding to the STAT3 SH2 domain. It is a very useful tool for exploring STAT3-mediated signaling pathways. It blocks LPS-mediated STAT3 tyrosine phosphorylation which leads to inhibition of LPS-mediated IL-1β and IL-6 production. In primary cultures of rat neural stem cells it blocks IL-15-induced differentiation.

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Cell-permeable pyrrazolopyrimidine derivative that inhibits AMP kinase (Ki=109nM in the absence of AMP) in an ATP-competitive manner. It displays no significant inhibition of ZAPK, SYK, PKCT, PKA and JAK3. Decreases food intake in mice and inhibits the effects of AICAR and metformin. It has been shown to inhibit BMP type I receptors ALK2, ALK3 and ALK6. Promotes cardiomyogenesis in mouse embryonic stem cells. Induces protective autophagy in cancer cell lines.

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A potent and selective inhibitor of insulin-like growth factor 1 receptor (IGF-1R, IC50=6 nM). It efficiently blocks IGF-1R activity, reduces pAKT and pERK1/2, induces apoptosis in IGF-1R-positive tumor cells and causes complete tumor regression in xenografted and allografted mice. Picropodophyllin downregulates IGF-1R by interfering with the action of β-arrestin 1/MDM2. It causes tumor regression and attenuates invasiveness of uveal melanoma cells. It displays beneficial effects on tumor growth, angiogenesis, bone disease and survival in a mouse multiple myeloma model. It attenuates intimal hyperplasia after vascular injury. Inhibition of IGF-1R is non-competitive with ATP.

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Jnk inhibitor

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A selective inhibitor of MEK1 and MEK2 (IC50= 0.18 and 0.22 µM respectively). Displays neuroprotective effects against ischemic brain injury.

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Alpha-crystallins composed of alpha A (~20 kDa) and alpha B (~20 kDa) subunits, are major water-soluble proteins accounting for almost 50% of total protein in the mammalian transparent eye lens and they are also found in a variety of other tissues. The two other crystallin families, beta and gamma, are homologous to each other but not to the alpha family or the sHsp's. Alpha-crystallins are also referred to as small heat shock proteins, since they are induced by increased temperature in a variety of organisms. The alpha-crystallins have sequence homology as well as structural and functional similarities with the small Hsp's such as Hsp25/27. Most small heat shock proteins have four common structural and functional features: (i) molecular weight between 12 and 43kDa; (ii) the formation of large oligomeric complexes composed of alphaA-crystallin, alphaB-crystallin and Hsp25/27; (iii) the moderately conserved alpha-crystallin domain in the central region of the protein; and (iv) molecular chaperone activity. The alpha-crystallin domain comprises approximately 90 residues, is bounded by variable N-terminal and C-terminal extensions and is involved in oligomer assembly. Oligomers can reach 800kDa or more and are dynamic, exhibiting subunit exchanges and organizational plasticity, possibly leading to functional diversity. Phosphorylation of serine residues occurs during development and in response to stress, and usually decreases oligomer size. Chaperone activity requires, and is modulated by, oligomerization and is limited to binding unfolded intermediates to prevent irreversible aggregation, although productive release and refolding of denatured proteins requires close cooperation with other chaperones. Other proposed functions include a role in membrane stabilization and modulation of intermediate filament organization during physiological stress and neurodegenerative disease.

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Concentrated lysis buffer for preparation of cell and tissue extracts.

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Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.

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DnaK possesses an autophosphorylation activity and a weak 5-nucleotidase activity, cleaving the 5 phosphate groups from both ribose and deoxyribose nucleotides. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E. coli physiology, where, like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes.

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Supersensitive spin label

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Topoisomerase I inhibitor