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172061 Résultats pour : "Blotting"

Experimentation kits, dot blot analysis

Experimentation kits, dot blot analysis

Supplier: G-Biosciences

Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.

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picoLUCENT™ PLUS-AP kit

picoLUCENT™ PLUS-AP kit

Supplier: G-Biosciences

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with alkaline phosphatase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

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Protein ladders (pre-stained), ScanLater™

Protein ladders (pre-stained), ScanLater™

Supplier: Molecular Devices

The ScanLater™ Western blot protein ladder is a mixture of seven biotinylated recombinant proteins at 10, 20, 40, 60, 80, 100, and 140 kD.

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Chemiluminescent acridinium esters

Chemiluminescent acridinium esters

Supplier: ENZO LIFE SCIENCES

Chemiluminescent acridinium esters are commonly used to label antibodies, proteins, nucleic acids, and some peptides, and are frequently used as detection reagents in immunoassays.

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Millipore® Immobilon®-NC Hydrophilic Transfer Membrane

Millipore® Immobilon®-NC Hydrophilic Transfer Membrane

Supplier: Merck

Triton-free, mixed cellulose esters transfer membrane.

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VWR®, Papier buvard, grade 703

VWR®, Papier buvard, grade 703

Supplier: VWR Collection

Idéal comme mèches pour le buvardage de protéines et d’acides nucléiques. Fournit un flux uniforme de tampon à travers le gel vers la membrane de transfert dans un sandwich de buvardage. Convient également pour retirer les gels sur les supports en verre.

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Millipore® Immobilon®-PSQ NOW Bundle Hydrophobic PVDF Transfer Membrane

Millipore® Immobilon®-PSQ NOW Bundle Hydrophobic PVDF Transfer Membrane

Supplier: Merck

PVDF transfer membrane hydrophobic for western blotting using chemiluminescence, chromogenic or radioactive detection methods.

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VWR®, Tampons buvard, grade 707

VWR®, Tampons buvard, grade 707

Supplier: VWR Collection

Ce papier très épais complète le sandwich de blotting. Sa texture lisse et régulière assure un contact continu entre le tampon de la chambre, de transfert, le gel et la membrane de transfert.

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Solution RapidBlock™, 10X

Solution RapidBlock™, 10X

Supplier: VWR Chemicals

La solution RapidBlock™ réduit le temps de blocage à 5 minutes pour les procédures de transfert Western et de « dot blot » sur membranes en PVDF et nitrocellulose. Cette formule sans protéines minimise la réactivité croisée et les liaisons aux anticorps non spécifiques, en produisant des transferts avec un bruit de fond faible et un meilleur rapport signal/bruit.

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Gel documentation system, G:BOX Mini

Gel documentation system, G:BOX Mini

Supplier: Syngene (a division of the Synoptics group)

The G:BOX mini is a compact, multi application imaging system for accurately imaging mini and midi fluorescence and visible gels, multiplex fluorescence Western blots, stain free gels and chemiluminescent blots.

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Anti-IgG (H+L) Goat Polyclonal Antibody (Peroxidase)

Supplier: Rockland Immunochemicals

Anti-Golden Syrian Hamster IgG whole molecule antibody is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays.

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Western ReProbe™ PLUS

Western ReProbe™ PLUS

Supplier: G-Biosciences

Western ReProbe™ PLUS allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies.

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Sondes fluorescentes, substrat de caspase-3 NucView™

Sondes fluorescentes, substrat de caspase-3 NucView™

Supplier: Biotium

Les substrats NucView® caspase-3 sont des sondes fluorescentes qui permettent la détection en temps réel de l’apoptose en surveillant l’activité de la caspase-3/7 dans des cellules intactes à l’aide de la microscopie confocale, de la cytométrie en flux ou de lecteurs de microplaques.

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Millipore® Immobilon®-E NOW Bundle Hydrophobic PVDF Transfer Membrane

Millipore® Immobilon®-E NOW Bundle Hydrophobic PVDF Transfer Membrane

Supplier: Merck

The first PVDF transfer membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.

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Tampon SSC, 20X, ultrapure

Tampon SSC, 20X, ultrapure

Supplier: VWR Chemicals

Le tampon SSC est un tampon d'hybridation pour les transferts Northern et Southern.

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3-Hydroxy-4-(2-sulfonato-4-(4-sulfonatophénylazo)phénylazo)naphtalène-2,7-disulfonate de tétrasodium solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Ponceau S solution has been used for membrane staining (Western blotting).

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Anti-IgG (H+L) Donkey Polyclonal Antibody

Anti-IgG (H+L) Donkey Polyclonal Antibody

Supplier: Rockland Immunochemicals

Anti-Rabbit IgG (H&l) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

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Anti-AKT1 Mouse Polyclonal Antibody (Unconjugated) [clone: 17F6.B11]

Anti-AKT1 Mouse Polyclonal Antibody (Unconjugated) [clone: 17F6.B11]

Supplier: Rockland Immunochemicals

This monoclonal antibody is suitable for ELISA, immunochemistry, immunoprecipitation and western blotting.  Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT Protein by western blotting in the appropriate cell lysate.

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Anti-UBB Rabbit Polyclonal Antibody

Anti-UBB Rabbit Polyclonal Antibody

Supplier: Rockland Immunochemicals

This purified polyclonal antibody reacts with ubiquitin by ELISA and western blot. Although not tested, this antibody is likely functional in immunochemistry and immunoprecipitation. For detection of free Ub by western blotting use Tris-Tricine SDS-PAGE.

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Restriction enzymes, Dpn I restriction enzymes

Supplier: AGILENT

Dpn I is an ideal enzyme for cloning and southern blot analysis.

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Anti-SH3BP2 Rabbit Polyclonal Antibody

Anti-SH3BP2 Rabbit Polyclonal Antibody

Supplier: Rockland Immunochemicals

This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 60 kDa in size corresponding to SH3BP2 by western blotting.

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Anti-PDPK1 Rabbit Polyclonal Antibody

Anti-PDPK1 Rabbit Polyclonal Antibody

Supplier: Rockland Immunochemicals

This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band ~60 kDa in size corresponding to human PDK-1 Protein by western blotting.

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Anti-IgG gamma Goat Polyclonal Antibody (Biotin)

Anti-IgG gamma Goat Polyclonal Antibody (Biotin)

Supplier: Rockland Immunochemicals

Anti-Monkey IgG (gamma chain) biotin conjugated is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays.

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Anti-LDB1 Rabbit Polyclonal Antibody

Anti-LDB1 Rabbit Polyclonal Antibody

Supplier: Rockland Immunochemicals

This affinity purified antibody has been tested for use in ELISA, western blot and CHIP.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB1 by western blotting.

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Anti-MTOR Rabbit Polyclonal Antibody

Anti-MTOR Rabbit Polyclonal Antibody

Supplier: Rockland Immunochemicals

This affinity purified antibody has been tested for use in immunochemistry, ELISA and western blotting. Western blotting shows reactivity specific for phospho mTOR detecting a band at approximately 250 kDa.

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Semi-dry transfer unit, TE77X

Semi-dry transfer unit, TE77X

Supplier: HOEFER ELECTROPHORESIS

Semi-dry transfer unit, TE77X

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Anti-GADD45G Rabbit Polyclonal Antibody

Anti-GADD45G Rabbit Polyclonal Antibody

Supplier: Rockland Immunochemicals

This antibody has been tested for use in ELISA, western blotting and immunoprecipitation.  Reactivity in other immunoassays is unknown. This product was assayed by western blotting after immunoprecipitation of mouse GADD45 gamma from a lysate.

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Anti-ANGPT1 Rabbit Polyclonal Antibody

Anti-ANGPT1 Rabbit Polyclonal Antibody

Supplier: Rockland Immunochemicals

Suitable for western blotting, Immunohistochemistry (IHC) and other antibody based assays.  A 1:500 dilution is recommended for western blotting.  The reaction of this antiserum directly with cell supernatants may result in high background due to reactivi

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Anti-IgG F(ab')2 Rabbit Polyclonal Antibody (Peroxidase)

Anti-IgG F(ab')2 Rabbit Polyclonal Antibody (Peroxidase)

Supplier: Rockland Immunochemicals

Anti-Chicken IgG F(ab')2 is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

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Anti-IgG (H+L) Donkey Polyclonal Antibody (Biotin)

Anti-IgG (H+L) Donkey Polyclonal Antibody (Biotin)

Supplier: Rockland Immunochemicals

Anti-Mouse IgG whole molecule is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

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