Affinity chromatography columns, HiTrap™ Benzamidine FF
HiTrap™ Benzamidine FF (High Sub), PP, 90 µm, 16×25 mm, 5 ml, <20 ml/min
17-5144-01
: Cytiva
HiTrap Benzamidine FF (high sub) are prepacked with Benzamidine Sepharose 4 Fast Flow (high sub) and designed specifically for the removal and/or purification of serine proteases such as thrombin.
- Effective removal of thrombin and factor Xa after tag cleavage of recombinant proteins
- Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates
- High binding capacity
- It can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA design or FPLC system
HiTrap Benzamidine FF (high sub) are prepacked 1 ml and 5 ml columns for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
The medium, Benzamidine Sepharose 4 Fast Flow (high sub), is also available as lab packages and is an excellent choice for scale-up.
- Débit:<20 ml/min
- Granulométrie:90 µm
- Capacité:5 ml
- Désignation:HiTrap™ Benzamidine FF (High Sub)
- Seq:03
- Matériau de colonne:PP
- Dimensions du lit:16×25 mm
- Plage de pH:2 - 8
- Cat. No.:17-5144-01
- Stockage:2 to 8 °C, 20% Ethanol, 0,05 M Sodium Acetate, pH 4,0
- Pression max.:5 bar
Méthanol ≥99.8%, HiPerSolv CHROMANORM® Reag. Ph. Eur., grade Gradient pour HPLC
#: 20864.320
: VWR Chemicals
Acétonitrile ≥99.9%, HiPerSolv CHROMANORM® pour LC-MS, pour les systèmes UPLC/UHPLC/Ultra HPLC
#: 83640.320
: VWR Chemicals
Sodium hydroxyde 0.1 mol/l (0.1 N) en solution aqueuse, AVS TITRINORM Reag. Ph. Eur. solution volumétrique
#: 31770.294
: VWR Chemicals
Eau, HiPerSolv CHROMANORM® pour LC-MS, pour les systèmes UPLC/UHPLC/Ultra HPLC
#: 83645.320
: VWR Chemicals
Solutions tampon, Certipur®, prêtes à l'emploi, température de référence à +20 °C, Supelco®
#: 1.09439.1000
: Merck