- Cdt:20 µG
- Type d'enzyme:Recombinant
- Source:Insect cells (Sf9)
- Species:Human
- Conditions de stockage:May be stored at 4 °C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at −20 °C. Aliquots are stable for 6 months after receipt at −20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Formulaire:Supplied as a liquid in 16 mM HEPES, pH 7,5, 400 mM sodium chloride, 20% glycerol
- Conditions de transport:20
- Nom de l'enzyme:DNA topoisomerase I
- Purity:≥95% (SDS-PAGE)
- Molecular weight:91,58
- Concentration:~0,9 mg/ml
- N°:Highly purified
DNA topoisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription.
This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in Scleroderma patients (specificity 98 to 100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis.
In human tissues the DNA topoisomerase I is initially synthesised as a protein with 100 kDa molecular weight. Most of this precursor is then proteolytically processed to a species with 70 kDa molecular weight from which the Scl-70 antigen has derived its name.
Recombinant Human Scl-70 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 91576 Da. Recombinant Scl-70 is expressed with a 6× His tag and purified by proprietary chromatographic techniques.