Spécifications
- Cdt:96 T
- Durée du test:Multiple steps
- Type de test:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rabbit
- Cible:beta Actin
- Désignation:Rabbit beta Actin ELISA kit
- Type déchantillon:Serum, plasma, tissue homogenates, and other biological fluids
- Réactivité croisée:Rabbit beta Actin ELISA Kit exhibits high specificity and excellent specificity for the detection of rabbit beta Actin. No significant cross-reactivity or interference between beta Actin and analogues was observed.
- Méthode de détection:Colorimetric
- Time to Results:4 h 30 min
- Principe du test:Quantitative
- Durée de conservation:Store for 6 months at 4 °C
- Portée de détection:0,313 - 20 ng/ml
- Température de stockage:4 °C
- Volume d'échantillon:100 μl
- Sensibilité:0,188 ng/ml
- Regulatory status:RUO
Spécifications
À propos de cet article
Rabbit beta Actin ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rabbit beta Actin in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Rabbit beta Actin ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of rabbit beta Actin in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for beta Actin has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the beta Actin present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated anti-beta Actin antibody, which binds the captured beta Actin present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of beta Actin captured in each well. The concentration of beta Actin can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.