Specifications
- Cdt:96 Tests
- Durée du test:Multiple steps
- Type de test:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Cible:CD30
- Désignation:Human CD30 ELISA kit
- Type déchantillon:Serum, plasma, tissue homogenates and other biological fluids
- Réactivité croisée:Human CD30 ELISA kit exhibits high specificity and excellent specificity for the detection of human CD30. No significant cross-reactivity or interference between CD30 and analogues was observed.
- Méthode de détection:Colorimetric
- Time to Results:4 h 30 min
- Principe du test:Quantitative
- Durée de conservation:Store for 6 months at 4 °C
- Portée de détection:0,781 - 50 ng/ml
- Température de stockage:4 °C
- Volume d'échantillon:100 μl
- Sensibilité:0,469 ng/ml
- Regulatory status:RUO
Specifications
About this item
Human CD30 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human CD30 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Detection range: 0,781 to 50 ng/ml
- Sensitivity: 0,469 ng/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Human CD30 ELISA kit (A75046) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human CD30 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for CD30 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CD30 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-CD30 Antibody, which binds the captured CD30 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of CD30 captured in each well. The concentration of CD30 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.