Spécifications
- Cdt:96 Tests
- Durée du test:Multiple steps
- Type de test:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Cible:CGRP
- Désignation:Mouse CGRP ELISA kit
- Type déchantillon:Serum, plasma, tissue homogenates and other biological fluids
- Réactivité croisée:Mouse CGRP ELISA Kit exhibits high specificity and excellent specificity for the detection of mouse CGRP. No significant cross-reactivity or interference between CGRP and analogues was observed.
- Méthode de détection:Colorimetric
- Time to Results:4 h 30 min
- Principe du test:Quantitative
- Durée de conservation:Store for 6 months at 4 °C
- Portée de détection:15,625 - 1000 pg/ml
- Température de stockage:4 °C
- Volume d'échantillon:100 μl
- Sensibilité:9,375 pg/ml
- Regulatory status:RUO
Spécifications
À propos de cet article
Mouse CGRP ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse CGRP in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 15,625 to 1000 pg/ml
- Sensitivity: 9,375 pg/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Mouse CGRP ELISA Kit (A76318) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse CGRP in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for CGRP has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CGRP present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-CGRP Antibody, which binds the captured CGRP present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of CGRP captured in each well. The concentration of CGRP can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.