Mouse monoclonal IgG2a antibody against human, chimpanzee, common marmoset CD45RO, unconjugated.

• Leukocytes
• T cells
• T cells CD4
• T cells CD8

The UCHL1 antibody reacts with an extracellular epitope on CD45RO, which is the shortest isoform of CD45, a type I transmembrane glycoprotein and member of the protein tyrosine phosphatase family (receptor class 1/6 subfamily). Alternative splicing of exons 4, 5 and 6 that encode the extracellular RA, RB and RC polypeptides of CD45 gives rise to up to 8 isoforms with molecular masses of 180 - 240 kDa. Excision of all three exons generates the ~180 kDa CD45RO isoform, which is expressed on activated and memory (but not naïve) T cells, some B cell subsets, activated monocytes and macrophages, and granulocytes. CD45RO enhances both T cell receptor- and B cell receptor-mediated activation and is a known ligand for CD22 on B cells. The UCHL1 antibody has been used to identify T cell lymphomas and leukemia and is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. The proportion of CD45RO+ (memory) T cells typically increases with age. The UCHL1 epitope is destroyed by treatment with neuraminidase or O-glycosidase.

Type:
Antigen: CD45RO
Clonality: Monoclonal
Clone: UCHL1
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a kappa
Reactivity: Chimpanzee, Common marmoset, Human