DH5α Chemically Competent E. coli Cells
ORIGCC100003
: OriGene
New Product
- Désignation:DH5α chemically competent cells (≥10⁸ CFU/μg of pUC19 DNA)
- Environmentally Preferable:
- Conditions de stockage:The competent cells should be stored at −80 °C, pUC19 control DNA should be stored at −20 °C and SOC recovery medium should be stored at 4 °C immediately upon arrival
- Génotypes:F – φ80lacZDM15 D(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk–, mk+) phoA supE44 l – thi–1 gyrA96 relA1
- Caractéristique:Chemically competent accommodates small and larger plasmids RecA1 and endA1 markers for minimal recombination and enhanced quality of plasmid DNA
- Cdt:1 KIT
- lacZ marker for blue/white colour screening
- Transformation of cloned DNA into bacterial cells
- Ideal for subcloning and generating cDNA libraries
Kit contains:
DH5α chemically competent cells (5×200 μl)
Positive control DNA - pUC19 (50 pg/μl, 25 μl)
SOC medium: 20 ml