Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognises acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include Keratins 1, 3, 4, 5, 6, 8, 10, 14, 15, 16, and 19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRTL/KRTH is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.

Flow Cytometry: 0,5-1 µg/million cells in 0,1 ml Immunofluorescence: 1 - 2 µg/ml Immunohistochemistry (FFPE): 0,25-0,5 µg/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Optimal dilution of the pan Cytokeratin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6,0, for 10 - 20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimised for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Type: Primary
Antigen: pan
Clonality: Monoclonal
Clone: KRTL/1077 + KRTH/1076
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG
Reactivity: Human, Rat