- Cdt:1 KIT
- Type de test:Sandwich
- ELISA conjugué:HRP
- Hôte:Mouse
- Primary antibody reactivity:
- Cible:Apolipoprotein C-I
- Environmentally Preferable:
- Conditions de stockage:Store components at –20 °C (NOTE: exceptions below) for 6 months or until expiration date.
- Type déchantillon:Serum, plasma, tissue homogenates, cell culture supernates, other biological fluids
- Méthode de détection:Colorimetric, OD 450 nm
- Principe du test:Quantitative
- Specificity:Natural and recombinant Mouse Apolipoprotein C-I
- Sensibilité:1,57 ng/ml
Aviva Systems Biology APOC1 ELISA Kit (Mouse) (OKEH03072) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apoc1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Apoc1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apoc1 captured in well.
- Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Mean Intra-assay CV%: <= 7.9% (n = 20)
Mean Inter-assay CV%: <= 10.1% (n = 20)