Kits d'expérimentation, purification de l'enzyme de restriction EcoRI
Catalog # EDVO302
Supplier: EDVOTEK
Specifications
- Désignation:Purification de l'enzyme de restriction EcoRI
- Environmentally Preferable:
- Cdt:1 KIT
Specifications
About this item
In this experiment, students purify the restriction enzyme, Eco RI. This procedure utilised an ion exchange chromatograph step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.
- For 5 lab groups
- Complete in 3 hours
Autres éléments nécessaires: cuve d'électrophorèse, alimentation, système de visualisation UV, bain-marie, microcentrifugeuse, spectrophotomètre UV et cuvettes, micropipette automatique avec pointes, supports annulaires et pinces, et pipettes 10 ml.
Delivery: Kit includes instructions, ion exchange matrix, chromatography columns, E.coli cell extract, equilibrium and elution buffer, Lambda DNA, Lambda/Eco RI marker, KCl, glycerol, dilution and reaction buffers, gel loading solution, agarose, electrophoresis buffer, and stain.